Thermostable omega-transaminases
AssigneeCambrex Kariskoga AB
InventorsSachin Pannuri, Sanjay Venkatesh Kamat, Abraham Rogelio Martin-Garcia
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Abstract
Thermostable omega-transaminases, particularly thermostable omega-transaminases which have a high reaction rate and which are tolerant to high concentrations of donor amine, can be used to enrich enantiomerically a mixture of chiral amines or to synthesize stereoselectively one of a pair of chiral amines in which the amino group is bound to a non-terminal, chirally substituted, carbon atom.
Claims13
An isolated thermostable omega transaminase which comprises the amino acid sequence SEQ ID NO:16.
An isolated polynucleotide which encodes the amino acid sequence SEQ ID NO:16.
A method for enantiomeric enrichment of a mixture of two enantiomeric chiral amines comprising contacting:
- (a) a mixture comprising a first chiral amine and a second chiral amine which is an enantiomer of the first chiral amine, wherein the first chiral amine has the structural formula:
- and wherein the second chiral amine has the structural formula:
- wherein each of R1 and R2, when taken independently, is alkyl, arylalkyl, or aryl and may be unsubstituted or substituted with one or more enzymatically non-inhibiting groups, and wherein R1 and R2 render the molecule chiral;
- (b) a thermostable omega transaminase which comprises the amino acid sequence SEQ ID NO:16;
- and
- (c) an amino acceptor
- for a time sufficient to convert the first chiral amine to a ketone such that the amount of the second chiral amine in the mixture is at least about 90% relative to the amount of the first chiral amine.
A method for stereoselective synthesis of one of two chiral forms of an amine comprising contacting:
- (a) a ketone having the structural formula:
- wherein each of R1 and R2, when taken independently, is alkyl, arylalkyl, or aryl and may be unsubstituted or substituted with one or more enzymatically non-inhibiting groups;
- (b) a thermostable omega transaminase which comprises the amino acid sequence SEQ ID NO:16;
- and
- (c) an amino donor
- for a time sufficient to form a substantially greater amount of a first chiral form of the amine than the amount of a second chiral form of the amine.
Dependencies are inferred from the claim text.