Calcium binding recombinant antibody against protein C
AssigneeOklahoma Medical Research Foundation
InventorsAlireza Rezaie, Charles T. Esmon
Life of this patent
Estimating the term…
Abstract
A Ca2+ dependent recombinant antibody that specifically binds to a specific twelve peptide sequence (E D Q V D P R L I D G K) in the activation region of the Protein C has been constructed. The antibody does not bind to Activated Protein C and can be used to inhibit activation of Protein C by thrombin-thrombomodulin, in purification of Protein C, and in treatment of tumors.
Claims20
A recombinant Ca2+ dependent monoclonal antibody or antibody fragment including a heavy chain and a light chain, wherein the antibody or antibody fragment comprise the hypervariable regions of the monoclonal antibody produced by the hybridoma deposited with the American Type Culture Collection as ATCC No. HB 9892, where the recombinant monoclonal antibody or antibody fragment bind an epitope in the activation peptide region of the heavy chain of Protein C defined by EDQVDPRLIDGK (Sequence ID No. 1) and calcium ions, where the antibody and antibody fragment inhibit Protein C activation by thrombin-thrombomodulin, and wherein the antibody and antibody fragment are expressed in bacterial or insect cells.
A recombinant Ca2+ dependent monoclonal antibody or antibody fragment including a heavy chain and a light chain, wherein the antibody or antibody fragment comprise the hypervariable regions of the monoclonal antibody produced by the hybridoma deposited with the American Type Culture Collection as ATCC No. HB 9892, where the recombinant monoclonal antibody or antibody fragment bind an epitope in the activation peptide region of the heavy chain of Protein C defined by EDQVDPRLIDGK (Sequence No. 1) and calcium ions, where the antibody and antibody fragment inhibit Protein C activation by thrombin-thrombomodulin, and wherein the antibody and antibody fragment are humanized.
A method of making a recombinant Ca2+ dependent monoclonal antibody which binds an epitope in the activation peptide region of the heavy chain of Protein C defined by E D Q V D P R L I D G K (Sequence ID No. 1) and calcium ions, where the antibody inhibits Protein C activation by thrombin-thrombomodulin, by expressing nucleotide molecules encoding the hypervariable region of the heavy and light chains of the monoclonal antibody expressed by the hybridoma deposited with the American Type Culture Collection as ATCC No. HB 9892 in bacteria or insect cells.
A method of making a recombinant humanized Ca2+ dependent monoclonal antibody which binds an epitope in the activation peptide region of the heavy chain of Protein C defined by EDQVDPRLIDGK (Sequence No. 1) and calcium ions, where the antibody inhibits Protein C activation by thrombin-thrombomodulin, by expressing nucleotide molecules encoding the hypervariable region of the heavy and light chains of the monoclonal antibody expressed by the hybridoma deposited with the American Type Culture Collection as ATCC No. HB 9892 in bacteria or insect cells.
Dependencies are inferred from the claim text.