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US 7,247,451 B2Granted
Antithrombin protein and DNA sequences from black fly
AssigneeAuburn University
InventorsMary S. Cupp, Eddie W. Cupp
Life of this patent
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Abstract
The invention is drawn to anti-thrombin proteins from the salivary glands of the species, Simulium. Methods for recombinant production of the protein as well as biomedical uses are provided.
Claims8
A method of prophylaxis of thrombosis or thromboembolism in a mammal in need thereof, said method comprising administering to said mammal an effective amount of an anti-thrombin protein, wherein said protein comprises the amino acid sequence of SEQ ID NO:2.
A method of prophylaxis of thrombosis or thromboembolism in a mammal, said method comprising:
- (a) culturing a host cell comprising an isolated nucleic acid molecule which encodes an anti-thrombin protein having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:2 under conditions suitable to express said protein;
- and
- (b) administering to said mammal an effective amount of said protein isolated from said host cell or host cell culture supernatant with a volume of blood.
A pharmaceutical composition comprising:
- (a) a substantially purified protein having anti-thrombin activity, wherein said protein comprises the amino acid sequence of SEQ ID NO:2;
- (b) a plasminogen activator;
- and
- (c) a pharmaceutical carrier.
1 more element
Dependencies are inferred from the claim text.