Screening method for a substance having hypoglycemic activity by using STAT-induced inhibitor of STAT function-1
AssigneeTadamitsu Kishimoto
InventorsTadamitsu Kishimoto, Tetsuji Naka
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Abstract
The present invention discloses a screening method for a substance, which could be a therapeutic agent for diabetes with insulin resistance through regulating a function of a molecule involved in insulin signaling pathway. More specifically, the present invention discloses a screening method for a substance having hypoglycemic activity, which is characterized by contacting a sample to STAT-induced inhibitor of STAT function-1 in the presence of insulin, and by detecting inhibitory activity of STAT-induced inhibitor of STAT function-1 by a substance in the sample, as an index.
Claims6
A screening method for identifying a substance having STAT-induced inhibitor of STAT function-1 (SSI-1)-dependent hypoglycemic activity, comprising:
- (a) providing cells which express SSI-1 and cells which do not express SSI-1;
- (b) contacting a candidate substance with said cells each cultured in the presence of insulin;
- (c) assessing the cell proliferation rate of said cells;
- and
- (d) comparing the effect of the candidate substance on the cell proliferation rate of the cells which express SSI-1 with the effect of the candidate substance on the cell proliferation rate of the cells which do not express SSI-1, thereby determining whether said candidate substance has SSI-1 dependent hypoglycemic activity.
Dependencies are inferred from the claim text.