Method of manufacturing hydroxyapatite and uses therefor in delivery of nucleic acids
AssigneeCarnegie Mellon University
InventorsPrashant N. Kumta, Charles Sfeir, Jeffrey Hollinger, Daiwon Choi, Lee Weiss, Phil Campbell
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Abstract
Provided is a method for production of nanocrystalline hydroxyapatite particles, and nanocrystalline hydroxyapatite particles produced according to the method. The nanocrystalline hydroxyapatite particles exhibit substantially superior cell transformation abilities as compared to known and commercially-available calcium phosphate kits. The nanocrystalline hydroxyapatite particles also find use in tissue engineering applications, for example bone and tooth engineering and repair applications.
Claims34
A method for making nanocrystalline hydroxyapatite, comprising the step of reacting calcium ions with phosphate ions in the presence of hydroxyl ions to form calciumphosphate, wherein the ratio of calcium ions to phosphate ions in the reaction is greater than 167.
A method for introducing a biomolecule into a cell, comprising the step of contacting a cell with a composition comprising nanocrystalline hydroxyapatite prepared according to the step of reacting calcium ions with phosphate ions in the presence of a biomolecule and hydroxyl ions, at a calcium ion to phosphate ion ratio of greater than 167.
A method for treating a bone or tooth injury comprising the step of introducing into a site of injury in a bone or tooth a matrix containing a na nocrystailine hydroxyapatite complex comprising the nanocrystalli ne hyd roxyapatite complexed with a biomaterial, wherein the nanocrystalline hydroxyapatite is prepared by reacting calcium ions with phosphate ions in the presence of hydroxyl ions, wherein the ratio of calcium ions to phosphate ions is greater than 167.
A method for making hydroxyapatite comprising the step of reacting calcium ions with phoshate ions in the presence of hydroxyl ions, wherein the ratio of calcium ions to phosnhate ions is greater than 1.67 wherein the hydroxyapatite is prepared accord ing to the formula:
- n(CaCl2)+6(Na3PO4)+2NaOH-→Ca10(PO4)6(OH)2+20NaCl+(n−10)CaCl2,
- wherein n is greater than 10, and further wherein the hdroxyapatite is precipitated by mixing a solution of calcium chloride with a solution of NaCl, KCl, Na3PO4, and a buffer adjusted to pH 7.5 with NaOH, wherein the solution of NaCl, KCl, Na3PO4 and a buffer adjusted to pH 7.5 with NaOH, additionally comprises a sugar.
Dependencies are inferred from the claim text.