Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
AssigneeBayer Pharmaceuticals Corporation
InventorsSham-Yuen Chan, Hsinyi Yvette Tang, Yiwen Tao, Yongjian Wu, Ruth Kelly
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Abstract
The present invention relates to a method for increased production of a secreted, recombinant protein product through the introduction of molecular chaperones in a mammalian host cell. The present invention also relates to a mammalian host cell with enhanced expression of a secreted recombinant protein product by coexpressing at least one chaperone protein.
Claims7
A mammalian host cell for enhanced expression of a recombinant protein product, said mammalian host cell having genetic material coding for expression of said recombinant protein product and transformed with at least one expression vector comprising DNA encoding Hsp70 chaperone protein, wherein the DNA encoding Hsp70 chaperone protein consists of the sequence of SEQ ID NO:
- 13 and wherein said mammalian host cell expresses said recombinant protein product at an enhanced level.
Dependencies are inferred from the claim text.