Microorganisms and processes for enhanced production of pantothenate
AssigneeBASF SE
InventorsR. Roger Yocum, Thomas A. Patterson, Janice G. Pero, Theron Hermann
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Abstract
The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
Claims34
A process for the enhanced production of pantothenate, comprising culturing a microorganism having a methylenetetrahydrofolate (MTF) biosynthetic enzyme encoded by a Bacillus serA and/or a Bacillus glyA gene overexpressed, under conditions such that pantothenate production is enhanced.
A process for the enhanced production of pantothenate, comprising culturing a microorganism having
- (i) an increased level of a Bacillus pantothenate biosynthetic enzyme gene product selected from the group consisting of:
- ketopantoate hydroxymethyltransferase, ketopantoate reductase, pantothenate synthetase, and aspartate-α-decarboxylase, and
- (ii) an overexpressed methylenetetrahydrofolate (MTF) biosynthetic enzyme encoded by a Bacillus serA and/or a Bacillus glyA gene,
- under conditions such that pantothenate production is enhanced.
A process for the enhanced production of pantothenate, comprising culturing a microorganism overexpressing a Bacillus panB gene and having at least one methylenetetrahydrofolate (MTF) biosynthetic enzyme encoded by a gene selected from the group consisting of Bacillus serA, Bacillus serB, Bacillus serC, Bacillus mtrA, Bacillus sul, Bacillus fol, Bacillus pab, Bacillus gcv, and/or Bacillus glyA overexpressed, under conditions such that pantothenate production is enhanced.
A process for the enhanced production of pantothenate, comprising culturing a microorganism overexpressing a Bacillus panB gene and having at least one methylenetetrahydrofolate (MTF) biosynthetic enzyme encoded by a gene selected from the group consisting of Bacillus serA, Bacillus mtrA, Bacillus sul, Bacillus fol, Bacillus Bacillus pab, Bacillus gcv, and/or Bacillus glyA overexpressed, under conditions such that pantothenate production is enhanced.
A process for the enhanced production of pantothenate, comprising culturing a microorganism having
- (i) an increased level of a Bacillus pantothenate biosynthetic enzyme gene product selected from the group consisting of:
- ketopantoate hydroxymethyltransferase, ketopantoate reductase, pantothenate synthetase, and aspartate-α-decarboxylase, and
- (ii) an overexpressed methylenetetrahydrofolate (MTF) biosynthetic enzyme encoded by a gene selected from the group consisting of:
- Bacillus serA, Bacillus mtrA, Bacillus sul, Bacillus fol, Bacillus pab, Bacillus gcv, and/or Bacillus glyA,
- under conditions such that pantothenate production is enhanced.
Dependencies are inferred from the claim text.