Method of uncoupling the catabolic pathway of glycolysis from the oxidative membrane bound pathway of glucose conversion

A process for producing a product in a bacterial cell comprising,

• a) obtaining a recombinant bacterial cell which has been altered by uncoupling an oxidative productive pathway, wherein glucose is oxidized to gluconate from a catabolic metabolic pathway wherein the bacterial cell is selected from Erwinia, Enterobacter, Corynebacteria, Acetobacter, Bacillus, Gluconobacter, Pantoae, and Escherichla cells, said uncoupling comprising inactivating a polynucleotide encoding a glucokinase which phoaphorylates D-glucose at its 6th carbon and having at least 95% sequence identity to SEQ ID NO:
• 2,
• b) culturing the recombinant bacterial cell under conditions suitable for the production of a product in the presence of a carbon source comprising glucose, and
• c) producing the product,
• wherein the product is gluconuate, 2-keto-D-gluconate, 2,5-diketo-D-gluconate, or 2-keto-L-guionic, and wherein the production of the product is enhanced compared to the level of production of the same product in an unaltered bacterial cell under the same conditions.