Diterpene-producing unicellular organism
AssigneeWilliam Marsh Rice University
InventorsSeiichi P. T. Matsuda, Elizabeth A. Hart
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Abstract
The present invention is directed to a unicellular organism system, such as a yeast, for producing geranylgeranyl pyrophosphate and a diterpene in vivo. The yeast cell preferably comprises an inducible nucleic acid sequence encoding geranylgeranyl pyrophosphate synthase, an inducible nucleic acid sequence encoding a soluble form of HMG-CoA reductase, a nucleic acid sequence of an allele that confers an increase in sterol metabolic flux and, in the diterpene-producing cell, a diterpene synthase.
Claims69
A unicellular organism for producing a diterpene, comprising:
- a first exogenous nucleic acid having the sequence of SEQ ID NO:
- 1 and encoding a geranylgeranyl pyrophosphate synthase, the first nucleic acid under the control of a first promoter operable in said organism;
- and
- a second exogenous nucleic acid having the sequence of SEQ ID NO:
- 361 and encoding a diterpene synthase, the second nucleic acid under the control of a second promoter operable in said organism.
A unicellular organism for producing a diterpene or diterpene precursor, comprising:
- a first exogenous polynucleotide encoding a geranylgeranyl pyrophosphate synthase having the amino acid sequence of SEQ ID NO:
- 22, the first polynucleotide under the control of a first promoter in said organism;
- and
- a second exogenous polynucleotide encoding a diterpene synthase having the amino acid sequence of SEQ ID NO:
- 383, the second polynucleotide under the control of a second promoter operable in said organism.
Dependencies are inferred from the claim text.