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US 7,238,485 B2Granted
Methods and apparatus for characterizing polynucleotides
AssigneesPresident and Fellows of Harvard College, The Regents of the University of California, Agilent Technologies, Inc.
InventorsMark Akeson, Daniel Branton, David W. Deamer, Jeffrey R. Sampson
Life of this patent
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Abstract
Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
Claims5
A nanopore analysis system, comprising:
- (a) a structure having a nanopore aperture;
- and
- (b) a molecular motor adjacent the nanopore aperture, wherein the molecular motor is capable of moving a polynucleotide with respect to the nanopore aperture at a rate of 350–2000 Hz.
A nanopore analysis system, comprising:
- (c) a structure having a nanopore aperture;
- and
- (d) a molecular motor (i) comprising a DNA polymerase, a ribosome, an exonuclease, or a helicase and (ii) disposed adjacent the nanopore aperture, wherein the molecular motor is capable of moving a polynucleotide with respect to the nanopore aperture at a rate of 75–2000 Hz.
Dependencies are inferred from the claim text.