Column packing material
AssigneeSequant AB
InventorsWen Jiang, Knut Irgum
Life of this patent
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Abstract
A porous silica particle suitable can be used in zwitterionic high performance liquid chromatography. The particle comprises covalently bound zwitterionic groups grafted through a polymerization reaction. A hydrophilic column packing material suitable for use as a stationary phase in zwitterionic high performance liquid chromatography may comprise porous silica particles. The particles may comprise covalently bound zwitterionic groups. Methods for manufacturing and applying the silica particles exist.
Claims12
A porous silica particle for use in zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising zwitterionic groups that have been graft-polymerized on the particle.
A method for producing porous silica particles for zwitterionic and hydrophilic interaction high performance liquid chromatography, comprising:
- providing porous silica particles suitable for use in zwitterionic and hydrophiuic interaction high performance liquid chromatography;
- suspending the silica particles in an aqueous solution of a zwitterionic methacryloxyethyl monomer, thereby obtaining a suspension of silica particles;
- allowing said suspension to equilibrate at room temperature;
- heating said equilibrated suspension to a temperature at or between 40° C. and 70° C.;
- and
- adding an aqueous solution of ammonium cerium nitrate, thereby initiating graft-polymerization, wherein
- the porous silica particles comprise graft-polymerized zwitterionic groups.
Dependencies are inferred from the claim text.