System, device and method for determining the concentration of an analyte

An analyte sensing device for sensing a concentration of analyte in a fluid of an animal body, the analyte sensing device capable of being implanted in the animal body such that it is in communication with the fluid, the analyte sensing device comprising:

• a housing;
• and
• a sensor layer disposed within the housing, wherein the sensor layer having at least one optical property that is responsive to the concentration of analyte in communication therewith, the sensor layer includes:
• a matrix having a plurality of analyte equivalent residues disposed therein;
• at least one extra-matrix space disposed between the matrix and the housing;
• and
• a plurality of analyte binding receptor molecules, wherein the analyte binding receptor molecules are capable of reversibly binding with the analyte equivalent residues and the analyte;
• and
• wherein the housing includes a permeable membrane that is permeable to analyte and non-permeable to the sensor layer and wherein the at least one optical property includes a scattering of optical radiation incident on the sensor layer.

A method to detect the concentration of an analyte in a fluid using an analyte sensing device comprising a sensor layer disposed within the housing, wherein the sensor layer exhibits at least one optical property that is responsive to the concentration of analyte in communication therewith, the sensor layer including a matrix having a plurality of analyte equivalent residues disposed therein, and a plurality of analyte binding receptor molecules, wherein the analyte binding receptor molecules are capable of reversibly binding with the analyte equivalent residues and the analyte, the method comprising:

• placing an analyte sensing device in communication with the fluid;
• providing incident optical radiation to the analyte sensing device;
• receiving responsive optical radiation from the analyte sensing device;
• determining the concentration of analyte by using the responsive optical radiation to measure a change in scattering of optical radiation by the sensor layer;
• and
• outputting data which is representative of the concentration of analyte.

An analyte sensing system comprising:

• analyte sensing device to sense a concentration of analyte in a fluid, the analyte sensing device including:
• a first sensor layer having at least one optical property that is responsive to the concentration of analyte in communication therewith, the first sensor layer including:
• a matrix having a plurality of first analyte equivalent residues disposed therein;
• and
• a plurality of first analyte binding receptor molecules, wherein the first analyte binding receptor molecules are capable of reversibly binding with the first analyte equivalent residues and the analyte;
• wherein the at least one optical property includes a scattering of optical radiation incident on the first sensor layer;
• and
• means for measuring the at least one optical property to determine the concentration of analyte in communication with the first sensor layer.

An analyte sensing system comprising:

• an analyte sensing device, capable of being implanted in an animal body such that it is in communication with a fluid in the animal body, the analyte sensing device comprising:
• a housing;
• and
• a sensor layer disposed within the housing, wherein the sensor layer having at least one optical property that is responsive to the concentration of analyte in communication therewith, the sensor layer includes:
• a matrix having a plurality of analyte equivalent residues disposed therein;
• at least one extra-matrix space disposed between the matrix and the housing;
• and
• a plurality of analyte binding receptor molecules, wherein the analyte binding receptor molecules are capable of reversibly binding with the analyte equivalent residues and the analyte;
• and
• wherein the housing includes a permeable membrane that is permeable to analyte and non-permeable to the sensor layer and wherein the at least one optical property includes a scattering of optical radiation incident on the sensor layer;
• and
• means for measuring the at least one optical property to determine the concentration of analyte in communication with the sensor layer.