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US 7,235,369 B2Granted
Enzyme-protein complex
AssigneeNichirei Biosciences Inc.
InventorsHirokazu Ohbayashi, Yuriko Kitano
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Abstract
A complex of enzyme, protein and carrier comprising two or more molecules of an enzyme conjugated through an amino group or other group to a carrier such as polylysine, and a protein (for example, antibody) with a specific binding potency to other substance(s) (for example, antigen) conjugated to at least one of said two or more molecules of the enzyme. This complex enables accurate assay of a trace amounts of a substance at a high sensitivity.
Claims22
A product comprising a carrier-enzyme-protein complex which comprises:
- a water-soluble carrier;
- an enzyme, two or more molecules of the enzyme being conjugated to the carrier;
- and
- a protein, which is conjugated to said enzyme, but not conjugated directly to the water-soluble carrier,
- wherein said protein is free to bind to at least one substance selected from the group consisting of an antigen, an antibody, an antibody fragment, a sugar chain, hyaluronic acid and biotin,
- wherein said protein is directly conjugated to at least one molecule of the two or more molecules of the enzyme.
3 more elements
A method for making a carrier-enzyme-protein complex, comprising:
- contacting a water soluble carrier, an enzyme and a protein,
- wherein said protein is free to bind to at least one substance selected from the group consisting of an antigen, an antibody, an antibody fragment, a sugar chain, hyaluronic acid and biotin, under conditions suitable for attachment of the carrier directly to the enzyme and suitable for attachment of the protein directly to the enzyme and
- recovering or isolating a complex of carrier-enzyme-protein;
- wherein said carrier-enzyme-protein complex comprises:
- a water-soluble carrier;
- an enzyme, two or more molecules of the enzyme being conjugated to the carrier;
- and a protein, which is not directly conjugated to said water-soluble carrier, and which is free to bind to at least one substance selected from the group consisting of an antigen, an antibody, an antibody fragment, a sugar chain, hyaluronic acid and biotin, wherein said protein is directly conjugated to at least one molecule of the two or more molecules of the enzyme.
4 more elements
Dependencies are inferred from the claim text.