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US 7,232,679 B2Granted
Isoprenoid production
AssigneeDsm IP Assets B.V.
InventorsAlan Berry, Werner Bretzel, Markus Hümbelin, Rual Lopez-Ulibarri, Anne Françoise Mayer, Alexei A. Yeliseev
Life of this patent
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Abstract
Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided.
Claims10
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 47;
- an amino acid sequence of a fragment of SEQ ID NO:
- 47 having hydroxymethylglutaryl-CoA synthase (HMG-CoA synthase) activity;
- an amino acid sequence of a polypeptide having HMG-CoA synthase activity, which is encoded by a polynucleotide that hybridizes to the nucleic acid sequence of SEQ ID NO:
- 46 under the following conditions:
- hybridization in 40% formamide, 1M NaCl, 1% sodium dodecyl sulfate (SDS) at 37° C., followed by at least one wash in 0.2×SSC at 60° C. for 20 minutes;
- and an amino acid sequence of a polypeptide having HMG-CoA synthase activity, which is at least 95% identical to SEQ ID NO:
- 47.
6 more elements
An isolated polypeptide comprising an amino acid sequence of a fragment of SEQ ID NO:
- 47, which polypeptide has HMG-CoA synthase activity.
Dependencies are inferred from the claim text.