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US 7,232,666 B2Granted
Optimized bacterial host strains of Methylomonas sp. 16a
AssigneeE. I. Du Pont de Nemours and Company
InventorsPamela L. Sharpe, Qiong Cheng, Melissa D. Bosak, Luan Tao, Natalia Sedkova
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Abstract
Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, ald, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented C30 carotenoid compounds, and show an increase in the production of C40 carotenoids. The use of the optimized host strains for the production of the C40 carotenoids canthaxanthin and astaxanthin is also described.
Claims7
A high growth methanotrophic bacterial strain which:
- a) grows on a C1 carbon substrate selected from the group consisting of methanol and methane;
- b) comprises a functional Embden-Meyerhof carbon pathway, said pathway comprising a gene encoding a pyrophosphate dependent phosphofructokinase enzyme;
- c) possesses a disruption such that at least one endogenous gene selected from the group consisting of carotenoid gene N1 (crtN1), aldehyde dehydrogenase gene (ald), carotenoid gene N2 (crtN2), and carotenoid gene N3 (crtN3) is down-regulated;
- and
- d) comprises a 16s rRNA sequence as set forth in SEQ ID NO:1.
2 more elements
Dependencies are inferred from the claim text.