Arrayed biomolecules and their use in sequencing
AssigneeSolexa Limited
InventorsShankar Balasubramanian, David Klenerman, Colin Barnes, Mark Allen Osborne
Life of this patent
Estimating the term…
Abstract
The invention is directed to a method for analysing genome wide variation in an individual. The method comprises randomly fragmenting the individual's genome and generating sequence reads of multiple bases on all fragments of the individual's genome, aligning the sequence reads generated with a known genomic reference sequence, and analysing variations between the sequence reads derived from the individual's genome and the known genomic reference sequence.
Claims9
A method for analysing genome wide variation in an individual comprising:
- i) randomly fragmenting a genome of said individual;
- ii) generating sequence reads of multiple bases on all fragments of said genome;
- iii) aligning the sequence reads with a known genomic reference sequence;
- and
- iv) analysing variations between the sequence reads derived from the genome of the individual and the known genomic reference sequence.
A method for analysing genome wide variation in an individual comprising:
- i) randomly fragmenting a genome of said individual;
- ii) arraying the fragments such that different fragments can be individually resolved by optical microscopy;
- iii) generating sequence reads of multiple bases on all fragments of said genome;
- iv) aligning said sequence reads with a known genomic reference sequence;
- and
- v) analysing variations between the sequence reads derived from the individual sample and the known genomic reference sequence.
Dependencies are inferred from the claim text.