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US 7,229,789 B1Granted
Methods and compositions for extracting proteins from cells
AssigneeN.V. Organon
InventorScot R. Shepard
Life of this patent
Estimating the term…
Abstract
A process is disclosed for releasing proteins from cells and/or inactivating viruses. In the process, a host cell containing a protein of interest is contacted with a solution of an effective amount of a detergent.
Claims19
A method of releasing a protein of interest from host cells comprising
- a. suspending the host cells in a buffer, and
- b. adding a detergent to the suspended cells wherein the detergent is an amphipathic charged amine or an amphipathic charged amine oxide, and wherein the detergent is in a concentrated form of at least 3 times the desired final concentration of the detergent after dilution in the buffer of step a.
A method of releasing a protein of interest from host cells comprising
- a. suspending the host cells in a buffer, and
- b. adding a detergent to the suspended cells, wherein the detergent is selected from the group consisting of:
- tributylphosphate, dimethyldecylamine, dimethyltridecylamine, dimethylundecylamine, dimethyldidecylamine, dimethyltetradecylamine, dimethylhexadecylamine, dimethyldecylamineoxide, dimethylundecylamineoxide, dimethyldidecylamineoxide, dimethyltetradecylamineoxide and dimethyltridecylamineoxide, and wherein the detergent is in a concentrated form of at least 3 times the desired final concentration of the detergent after dilution in the buffer of step a.
Dependencies are inferred from the claim text.