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AssigneeDNAVEC Research Inc.
InventorsKaio Kitazato, Tsugumine Shu, Hidekazu Kuma, Yasuji Ueda, Makoto Asakawa, Mamoru Hasegawa, Akihiro Iida, Fumino Tokito, Takahiro Hirata, Tsuyoshi Tokusumi, Makoto Inoue, Yumiko Tokusumi
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F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
A Sendai virus particle comprising a complex comprising (a) a negative-strand single-stranded RNA expressing NP, P, and L proteins and modified not to express at least one of F and HN proteins, wherein the RNA is derived from a Sendai virus, and (b) NP, P, and L proteins, wherein the Sendai virus particle, after introduction into a cell, is capable of replicating said RNA, but incapable of releasing infective viral particles and propagating to adjacent cells.
A DNA encoding (i) negative-strand single-stranded RNA expressing NP, P, and L proteins and modified not to express at least one of F and HN proteins, wherein the RNA is derived from a Sendai virus, or (ii) the complementary strand of the RNA, wherein a complex comprising the RNA and NP, P, and L proteins, after introduction into a cell, is capable of replicating said RNA, but incapable of releasing infective viral particles and propagating to adjacent cells.
A method for producing virus particles which, after introduction into a cell, are capable of replicating a negative-strand single-stranded RNA comprised in the virus particles, but incapable of releasing infective viral particles and propagating to adjacent cells, comprising the following steps of:
A method for producing virus particles which, after introduction into a cell, are capable of replicating a negative-strand single-stranded RNA comprised in the virus particles, but incapable of releasing infective viral particles and propagating to adjacent cells, comprising the steps of:
A cell comprising a Sendai virus comprising a complex comprising:
A ribonucleoprotein complex comprising:
A cell comprising a ribonucleoprotem complex comprising:
Dependencies are inferred from the claim text.