Phospholipases, nucleic acids encoding them and methods for making and using them
AssigneeDiversa Corporation
InventorsSvetlana Gramatikova, Geoff Hazlewood, David Lam, Nelson R. Barton
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Abstract
The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.
Claims37
An isolated, synthetic or recombinant phospholipase (a) encoded by a phospholipase-encoding nucleic acid having at least 90% sequence identity to SEQ ID NO:1;
- (b) having a sequence comprising enzymatically active fragments of (a);
- (c) having the sequence of (a) or (b) and lacking an endogenous signal sequence;
- (d) having the sequence of (a), (b) or (c) and further comprising a heterologous signal sequence;
- or (e) having the sequence of (a), (b), (c) or (d) and further comprising a heterologous signal sequence,
- and optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection.
An isolated, synthetic or recombinant polypeptide having a phospholipase activity and (i) having at least 90% sequence identity to SEQ ID NO:2 wherein optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection, or, (ii) encoded by a nucleic acid having at least 90% sequence identity to the sequence of SEQ ID NO:1, and optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection, or
- (iii) having the sequence of (i) or (ii) and lacking an endogenous signal sequence;
- or
- (iv) having the sequence of (i), (ii) or (iii) and further comprising a heterologous signal sequence;
- or
- (v) having the sequence of (i), (ii), (iii) or (iv) and further comprising a heterologous signal sequence.
An isolated, synthetic or recombinant polypeptide having a phospholipase activity and (a) comprising a sequence having at least 90% sequence identity to at least 100 contiguous amino acid residues of SEQ ID NO:2;
- (b) having the sequence of (a) and lacking an endogenous signal sequence;
- (c) having the sequence of (a) or (b) and further comprising a heterologous signal sequence;
- or (d) having the sequence of (a), (b) or (c) and further comprising a heterologous signal sequence.
Dependencies are inferred from the claim text.