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US 7,217,544 B2Granted
Method for the preparation of L-amino acids from D-amino acids
AssigneeDegussa AG
InventorsWerner Hummel, Birgit Geueke, Steffen Osswald, Christoph Weckbecker, Klaus Huthmacher
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Abstract
The invention relates to recombinant microorganisms which, in comparison to the starting organism, have a higher concentration or activity of a D-amino acid oxidase, of an L-amino acid dehydrogenase, of an NADH cosubstrate regenerating enzyme and, if necessary, of a catalase. The invention also includes methods for preparing L-amino acids from D-amino acids using of these microorganisms.
Claims15
A method for manufacturing an L-amino acid from a D-amino acid comprising:
- a) reacting a recombinant microorganism with said D-amino acid, wherein:
- i) said recombinant microorganism has been made by transforming a starting microorganism with one or more vectors comprising polynucleotides encoding the enzymes:
- D-amino acid oxidase, wherein said D-amino acid oxidase is the D-amino acid oxidase of A. protophormiae;
- L-amino acid dehydrogenase, wherein said L-amino acid dehydrogenase is the L-amino acid dehydrogenase of Bacillus cereus;
- and an enzyme that regenerates NADH, wherein said enzyme that regenerates NADH is the malate dehydrogenase from A. protophormiae;
- ii) relative to said starting microorganism, said recombinant microorganism overexpresses each of said enzymes;
- iii) the reaction is carried out under conditions in which each of said enzymes is active;
- and
- b) isolating said L-amino acid.
6 more elements
Dependencies are inferred from the claim text.