Methods and compositions for degradation of nitroaromatic and nitramine pollutants
AssigneeUniversity of Iowa Research Foundation
InventorsJerald L. Schnoor, Beniot Van Aken
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Abstract
The invention relates to novel Methylobacterium species that are capable of degrading nitroaromatic and nitramine compounds. Compositions, kits and methods of using the Methylobacterium species for the degradation of nitroaromatic and nitramine pollutants are provided. More specifically, compositions and methods for the degradation or bioremediation of nitroaromatic and nitramine explosives and explosive residues are provided.
Claims34
A method for degrading a nitroaromatic or nitramine compound to produce one or more degradation products, the method comprising contacting said compound with an isolated bacterium having all identifying characteristics of the Methylobacterium species strain BJ001 having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946, whereby one or more degradation products is produced.
An isolated Methylobacterium having all identifying characteristics of the Methylobacterium species strain BJ001 having ATCC Accession No. PTA-5125 and NCIMB Aeccession No. 13946.
An isolated Methylobacterium which is the Methylobacterium having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946.
A method of preparing Methylobacterium species strain BJ001 comprising:
- inoculating a sterile preparation of growth medium that supports the growth of Methylobacterium species with a cell of Methylobacterium species strain BJ001 of ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946 and
- incubating the preparation or growth medium to produce a preparation of said Methylobacterium species strain BJ001.
A composition comprising an isolated Methylobacterium having all identifying characteristics of the Methylobacterium species strain BJ001 having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946, and a high energy compound.
A composition comprising Methylobacterium species strain BJ001 having all identifying characteristics of ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946 said composition having a cell concentration of at least 1% by volume of said Methylobacterium.
A composition comprising
- an aqueous slurry of said material comprising a nitroaromatic or nitramine compound, and
- a Methylobacterium species having all identifying characteristics of Methylobacterium species strain BJ001 having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946,
- said aqueous slurry being 30% solid material (w/w).
A composition comprising a viable, dried Methylobacterium having all identifying characteristics of Methylobacterium species strain BJ001 having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946.
A kit for the degradation of a nitramine or nitroaromatic compound, the kit comprising a Methylobacterium having all identifying characteristics of the species having ATCC Accession No. PTA-5125 and NCIMB Accession No. 13946.
Dependencies are inferred from the claim text.