Immunostimulatory polynucleotide/immunomodulatory molecule conjugates
AssigneeThe Regents of the University of California
InventorsDennis A. Carson, Eyal Raz, Mark Roman
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Abstract
Immunostimulatory polynucleotide-immunomodulatory molecule conjugate compositions are disclosed. These compositions include a polynucleotide that is linked to an immunomodulatory molecule, which molecule comprises an antigen and may further comprise immunomodulators such as cytokines and adjuvants. The polynucleotide portion of the conjugate includes at least one immunostimulatory oligonucleotide nucleotide sequence (ISS). Methods of modulating an immune response upon administration of the polynucleotide-immunomodulatory conjugate preparation to a vertebrate host are also disclosed.
Claims36
A method of reducing IgE production in a mammal comprising administering an immunomodulatory composition to the mammal in an amount sufficient to reduce IgE production in the mammal, wherein the immunomodulatory composition comprises an immunomodulatory molecule, wherein said immunomodulatory molecule comprises an antigen and is conjugated to an immunostimulatory polynucleotide (ISS-PN), said ISS-PN comprising an immunostimulatory sequence (ISS) comprising the sequence 5′-cytosine, guanine-3′.
A method for reducing antigen-stimulated inflammation in a mammal comprising administering an immunomodulatory composition to the mammal in an amount sufficient to reduce inflammation stimulated by the antigen in the mammal, wherein the immunomodulatory composition comprises an immunomodulatory molecule, wherein said immunomodulatory molecule comprises an antigen and is conjugated to an immunostimulatory polynucleotide (ISS-PN), said ISS-PN comprising an immunostimulatory sequence (ISS) comprising the sequence 5′-cytosine, guanine-3′.
A method of suppressing a Th2 immune response in a mammal comprising an immunomodulatory composition in an amount sufficient to suppress the Th2 immune response, wherein the immunomodulatory composition comprises an immunomodulatory molecule, wherein said immunomodulatory molecule comprises an antigen and is conjugated to an immunostimulatory polynucleotide (ISS-PN), said ISS-PN comprising an immunostimulatory sequence (ISS) comprising the sequence 5′-cytosine, guanine-3′.
A method of stimulating a Th1 immune response in a mammal comprising administering to the mammal an immunomodulatory composition in an amount sufficient to stimulate the Th1 immune response, wherein the immunomodulatory composition comprises an immunomodulatory molecule, wherein said immunomodulatory molecule comprises an antigen and is conjugated to an immunostimulatory polynucleotide (ISS-PN), said ISS-PN comprising an immunostimulatory sequence (ISS) comprising the sequence 5′-cytosine, guanine-3′.
Dependencies are inferred from the claim text.