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US 7,208,290 B2Granted
Methods of co-expressing umami taste receptors and chimeric Gα15 variants
AssigneeSenomyx, Inc.
InventorsXiaodong Li, Hong Xu, Lena Staszewski, Jon Elliot Adler
Life of this patent
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Abstract
The invention provides a series of chimeric Gα15 protein variants that couple to certain GPCRs more efficiently than the native Gα15 protein. These chimeric Gα15 protein variants can be used to discover and analyze modulators of GPCRs (especially those that mediate taste perception).
Claims6
A method for expressing a functional umami taste receptor comprising co-expressing (i) full-length unmodified rat, murine, rodent, human T1R1 and T1R3 taste receptor sequence in a cell which further expresses (ii) a chimeric G protein which is identical to the amino acid sequence recited in SEQ ID NO:
- 2 except for the substitution of an aspartic amino acid residue for the glycine residue at position 66, and the substitution of the last 6 amino acids of SEQ ID NO:
- 2 with the hexapeptide sequence encoded by the nucleic acid sequence contained in SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.