Antibodies to non-shed Muc1 and Muc16, and uses thereof
AssigneeImmunoGen, Inc.
InventorsGillian Payne, Thomas Chittenden, Viktor Goldmakher, Philip Chun, Kristin Sneider-Mulready, Carol A. Vater
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Abstract
The present invention relates to antibodies, antibody fragments, conjugates of antibodies and antibody fragments with cytotoxic agents, and hybridomas producing the antibodies and antibody fragments, where the antibodies and antibody fragments recognize extracellular epitopes of plasma membrane proteins that are not released into the extracellular fluid, and to methods for the detection, monitoring and treatment of malignancies such as breast cancer and ovarian cancer using the antibodies, antibody fragments and conjugates.
Claims37
An isolated monoclonal antibody MJ-170 produced by hybridoma cell line MJ-170 on deposit with the American Type Culture Collection (ATCC) as accession number PTA-5286.
An isolated rnonoclonal antibody MJ-171 produced by hybridoma cell line MJ-171 on deposit with the ATCC as accession number PTA-5287.
An isolated monoclonal antibody MJ-172 produced by hybridoma cell line MJ-172 on deposit with the ATCC as accession number PTA-5288.
An isolated monoclonal antibody MJ-173 produced by hybridoma cell line MJ-173 on deposit with the ATCC as accession number PTA-5302.
A hybridoma cell line MJ-170 on deposit with the ATCC as accession number PTA-5286.
A hybridoma cell line MJ-171 on deposit with the ATCC as accession number PTA-5287.
A hybridoma cell line MJ-172 on deposit with the ATCC as accession number PTA-5288.
A hybridoma cell line MJ-173 on deposit with the ATCC as accession number PTA-5302.
An isolated antibody that specifically binds to a Muc1 peptide selected from the group consisting of:
- a) QLTLAFREGTINVHDVETQFN;
- (SEQ ID NO:8)
- b) QYKTEAASRYNLTISDVSVSD;
- (SEQ ID NO:9)
- c) FLQIYKQGGFLGLSNIKFRPG;
- (SEQ ID NO:10)
- and
- d) VPFPFSAQSGAGVPGWGIA.
- (SEQ ID NO:12)
An isolated antibody that specifically binds to a Muc16 peptide selected from the group consisting of:
- a) SSVLVDGYSPNRNEPLTGNS;
- (SEQ ID NO:14)
- b) TNYQRNKRNIEDALNQLFRN;
- (SEQ ID NO:15)
- c) FRNSSIKSYFSDCQVSTFRSV;
- (SEQ ID NO:16)
- d) SVPNRHHTGVDSLCNFSPLARRV;
- (SEQ ID NO:17)
- and
- e) DRVAIYEEFLRMTRNGTQLQNFTLDRSS.
- (SEQ ID NO:18)
A hybridoma that produces an antibody that specifically binds to a MUC1 peptide selected from the group consisting of:
- a) QLTLAFREGTINVHDVETQFN
- (SEQ ID NO:8);
- b) QYKTEAASRYNLTISDVSVSD
- (SEQ ID NO:9);
- c) FLQIYKQGGFLGLSNIKFRPG
- (SEQ ID NO:10);
- and
- d) VPFPFSAQSGAGVPGWGIA
- (SEQ ID NO:12).
A hybridoma that produces an antibody that specifically binds to a MUC16 peptide selected from the group consisting of:
- a) SSVLVDGYSPNRNEPLTGNS
- (SEQ ID NO:14);
- b) TNYQRNKRNIEDALNQLFRN
- (SEQ ID NO:15);
- c) FRNSSIKSYFSDCQVSTFRSV
- (SEQ ID NO:16);
- d) SVPNRHHTGVDSLCNFSPLARRV
- (SEQ ID NO:17);
- and
- e) DRVAIYEEFLRMTRNGTQLQNFTLDRSS
- (SEQ ID NO:18).
Dependencies are inferred from the claim text.