Method for reductive amination of a ketone using a mutated enzyme
AssigneeBioCatalytics, Inc.
InventorJ. David Rozzell, Jr.
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Abstract
Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
Claims3
A method for producing an amine from a target ketone, comprising:
- creating a mutated enzyme that catalyzes reductive amination of the target ketone;
- and
- providing the mutated enzyme in a reaction mixture comprising the target ketone under conditions sufficient to permit the formation of the corresponding amine to thereby produce the amine,
- wherein the ketone is not a 2-ketoacid.
Dependencies are inferred from the claim text.