# US 7,201,844 B1 · Composite matrices with interstital polymer networks > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,201,844 B1 - Document ID: US7201844B1 - Status: Granted - Kind: B1 - Application number: 10/251,653 - Filed: 2002-09-20 - Granted: 2007-04-10 - Earliest claimed: 2001-03-14 - Assignees: Hammen Corporation - Inventors: Richard F. Hammen; John P. Hammen - Web page: https://patentgrep.com/patent/US7201844B1 ## CPC - B01J 20/26: Synthetic macromolecular compounds - B01J 20/267: {Cross-linked polymers} - B01J 20/28026: {Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin} - B01J 20/3204: {Inorganic carriers, supports or substrates} - B01J 20/321: {consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds} - B01J 20/3212: {consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds} - B01J 20/3219: {involving a particular spacer or linking group, e.g. for attaching an active group} - B01J 20/327: {Polymers obtained by reactions involving only carbon to carbon unsaturated bonds} - B01J 20/3272: {Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds} - B01J 20/3274: {Proteins, nucleic acids, polysaccharides, antibodies or antigens} - B01J 20/3276: {Copolymers} - B01J 20/3278: {Polymers being grafted on the carrier} - B01J 20/3282: {Crosslinked polymers} - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS ## Abstract The present invention relates in general to the preparation and use of matrices having solid spaces, interstitial spaces and interstitial polymer networks. In particular, the interstitial polymer networks have utility in chemical and biochemical separations, solid phase synthesis, catalysis of chemical reactions, and immobilized enzyme reactors. The interstitial polymer networks in one embodiment comprise crosslinked polymers suspended in the interstitial spaces from and/or between solid particles. The matrices are characterized by high ligand and functional group density and by reversible high sorptive and binding capacity, and are substantially accompanied by a very low nonspecific adsorption or interaction with molecules such as proteins. Moreover, the matrices of the invention exhibit other characteristics highly desirable in chromatographic and catalytic applications, such as high physical rigidity, high ligand density, chemical stability, high ligand reactivity, and rapid exchange and reaction kinetics. ## Claims ### Claim 1 A matrix comprising solid particles and interstitial space comprising (a) a cross-linked interstitial polymer network covalently attached to said particles which forms an integrated contiguous network in said interstitial space that is permeable to liquids, is effectively a large pore polymer contained within the interstitial space of the matrix and is sufficiently thin or dilute so as to not act as a significant barrier to fluid flow, and (b) a member of a binding pair, wherein at least one of the monomers used to synthesize said network comprises a hydrophilic monomer and wherein said solid particles have an irregular shape. ### Claim 2 (depends on 1) The matrix of claim 1 wherein said interstitial polymer network further comprises a tether molecule that covalently links said interstitial polymer network to said particles. ### Claim 3 (depends on 1) The matrix of claim 1 wherein said solid support further comprises a blocking reagent. ### Claim 4 (depends on 1) The matrix of claim 1 wherein said solid particles comprise metal, metal oxide, resin or glass. ### Claim 5 (depends on 1) The matrix of claim 1 wherein said solid particles comprise silica. ### Claim 6 (depends on 1) The matrix of claim 1 wherein said member of said binding pair is a cationic or anionic moiety. ### Claim 7 (depends on 1) The matrix of claim 1 wherein said cross-linked interstitial polymer network comprises pores comprising at least one dimension of at least 100 nanometers. ### Claim 8 (depends on 1) The matrix of claim 1 wherein said cross-linked interstitial polymer network comprises pores comprising at least one dimension of at least 500 nanometers. ### Claim 9 (depends on 1) A separation device comprising the matrix of claim 1. ### Claim 10 (depends on 9) An apparatus comprising the separation device of claim 9. ### Claim 11 A matrix comprising solid particles and interstitial space comprising (a) a cross-linked interstitial polymer network covalently attached to said particles which forms an integrated contiguous network in said interstitial space that is permeable to liquids, is effectively a large pore polymer contained within the interstitial space of the matrix and is sufficiently thin or dilute so as to not act as a significant barrier to fluid flow, and (b) a reactive moiety, wherein at least one of the monomers used to synthesize said polymer network comprises a hydrophilic monomer wherein said particles have an irregular shape. ### Claim 12 (depends on 11) The matrix of claim 11 wherein said reactive moiety comprises a chemical catalyst, an enzyme or a chemical reagent. ### Claim 13 (depends on 11) The matrix of claim 11 wherein said interstitial polymer network further comprises a tether molecule that covalently links said interstitial polymer network to said particles. ### Claim 14 (depends on 11) The matrix of claim 11 wherein said solid support further comprises a blocking reagent. ### Claim 15 (depends on 11) The matrix of claim 11 wherein said solid particles comprise metal, metal oxide, resin or glass. ### Claim 16 (depends on 11) The matrix of claim 11 wherein said solid particles comprise silica. ### Claim 17 (depends on 11) The matrix of claim 11 wherein said member of said binding pair is a cationic or anionic moiety. ### Claim 18 (depends on 11) The matrix of claim 11 wherein said cross-linked interstitial polymer network comprises pores comprising at least one dimension of at least 100 nanometers. ### Claim 19 (depends on 11) The matrix of claim 11 wherein said cross-linked interstitial polymer network comprises pores comprising at least one dimension of at least 500 nanometers. ### Claim 20 (depends on 11) A separation device comprising the matrix of claim 11. ### Claim 21 (depends on 11) An apparatus comprising the separation device of claim 11.