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US 7,198,935 B1Granted
Infectious pestivirus pseudo-particles containing functional Erns, E1, E2 envelope proteins
AssigneeInstitut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsBirke Bartosch, Francois-Loic Cosset
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Abstract
The invention relates to the generation and the use of pestivirus pseudo-particles containing native functional E1, E2 envelope glycoproteins assembled onto retroviral core particles. These particles are highly infectious and constitute a valid model of pestivirus virion.
Claims4
A method for producing pestivirus-like particles ex vivo comprising the steps of:
- providing a first nucleic acid sequence comprising a packaging competent genome from a retrovirus;
- providing a second nucleic acid sequence comprising a cDNA encoding core proteins from said retrovirus;
- providing a third nucleic acid sequence comprising a cDNA encoding a polyprotein comprising successively a pestivirus core protein, and a Erns protein and/or pestivirus E1 protein and/or a pestivirus E2 protein, and optionally a pestivirus p7 protein;
- transfecting host cells with said nucleic acid sequences and maintaining the transfected cells in culture for sufficient time to allow expression of the cDNAs to produce structural proteins from pestivirus and retrovirus;
- and
- allowing the structural proteins to form virus-like particles which do not include the pestivirus core protein.
3 more elements
Dependencies are inferred from the claim text.