Mass spectrometer and reaction cell for ion-ion reactions
AssigneeBruker Daltonik GmbH
InventorsJochen Franzen, Evgenij Nikolaev
Life of this patent
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Abstract
The invention relates to a reaction cell for reactions between different types of ion species and a related mass spectrometer to analyze the ion products. The invention consists in an RF-operated straight ion guide with a side inlet, particularly suitable for reactions between positive and negative ion species, one ion species being fed in through the side inlet. Particularly favorable is an ion guide made up of a set of coaxial apertured diaphragms with a slight axial potential gradient. The reactions can be used for a fragmentation of multiply charged protein or peptide ions by electron transfer, or for the removal of excess charges of multiply charged biopolymer ions, for example.
Claims17
Reaction cell for reactions of positive with negative ions, comprising an RF ion guide for one species of ion with a lateral inlet system for the other species of ion.
Mass spectrometer for scanning reaction products from reactions of analyte ions from substances under analysis with reactant ions from reaction substances, the analyte ions and reactant ions being of different ion species, positively and negatively charged, the spectrometer comprising:
- (a) an ion source for generating analyte ions,
- (b) an ion source for generating reactant ions,
- (c) a reaction cell having an RE ion guide with a passage for the analyte ions and a lateral inlet for reactant ions, and
- (d) a mass analyzer for analyzing the reaction products.
A method for mass spectrometrically analyzing analyte ions, comprising
- (a) generating analyte ions in a first ion source,
- (b) generating reactant ions in a second ion source, wherein the analyte ions and the reactant ions are different ion species with opposite charge,
- (c) guiding the analyte ions through an RF ion guide,
- (d) introducing the reactant ions via a lateral inlet into the RF ion guide, while the analyte ions are inside the RF ion guide to facilitate reaction between the analyte and the reactant ions generating reaction products, and
- (e) analyzing the reaction products in a mass analyzer.
Dependencies are inferred from the claim text.