Polynucleotides and polypeptides that confer increased biomass and tolerance to cold, water deprivation and low nitrogen to plants
AssigneeMendel Biotechnology, Inc.
InventorsCai-Zhong Jiang, Jacqueline E. Heard, Oliver Ratcliffe, Robert A. Creelman, Jose Luis Riechmann, Volker Haake
Life of this patent
Estimating the term…
Abstract
The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. These properties include increased biomass and increased tolerance to cold, water deprivation, and low nitrogen conditions.
Claims18
A transgenic plant transformed with an expression vector comprising a polynucleotide sequence encoding a polypeptide having a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO:
- 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in said transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant.
A method for producing a transgenic plant having greater tolerance to water deprivation than a control plant, the method steps comprising:
- (a) providing an expression vector comprising:
- (i) a polynucleotide sequence encoding a polypeptide comprising a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO:
- 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in said transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant;
- and
- (ii) at least one regulatory element operably linked to the polynucleotide sequence, wherein said at least one regulatory element controls expression of the polynucleotide sequence in a target plant;
- (b) introducing the expression vector into at least one plant;
- and
- (c) selecting at least one transgenic plant that has greater tolerance to water deprivation than the control plant.
A method for increasing the tolerance of a plant to water deprivation, the method steps comprising:
- (a) providing an expression vector comprising:
- (i) a polynucleotide sequence encoding a polypeptide comprising a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO:
- 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in the transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant;
- and
- (ii) at least one regulatory element flanking the polynucleotide sequence, wherein said at least one regulatory element controls expression of the polynucleotide sequence in a target plant;
- (b) introducing the expression vector into a plant, thereby producing a transgenic plant;
- and
- (c) selecting a transgenic plant having greater tolerance to water deprivation than a control plant.
Dependencies are inferred from the claim text.