Methods for treating wound tissue and forming a supplemented fibrin matrix

A method for forming a supplemented fibrin matrix, the method comprising applying to wounded tissue a composition in the form of a dry powder comprising fibrinogen in an amount which forms a fibrin matrix in the presence of thrombin, Factor XIII, and Ca++,

• providing to the composition, thrombin, Factor XIII, Ca++ and aqueous solution in amounts which form a fibrin matrix in the presence of fibrinogen,
• wherein said aqueous solution is provided to the dry powder composition at the site of the wounded tissue,
• wherein said composition is free of collagen;
• further wherein said composition contains no added fibrinolysis inhibitor,
• further wherein the composition further comprises at least one supplement selected from the group consisting of:
• analgesics, anesthetics, antimicrobial compounds, antibodies, anticoagulants, anti-inflammatory compounds, antiproliferatives, cytokines, cytotoxins, chemotherapeutic drugs, growth factors, hormones, interferons, lipids or lipid-containing structures, polynucleotides or oligonucleotides, osteoinducers, polymers, polysaccharides, proteoglycans, polypeptides, protease inhibitors, steroids, vasoconstrictors, vasodilators, vitamins, nutritional minerals and stabilizers;
• and
• further forming a fibrin matrix when said fibrinogen is applied to said wounded tissue in the presence of thrombin, Factor XIII, and Ca++, said fibrin matrix covering and adhering to said wounded tissue.

A method for forming a fibrin matrix, said method comprising applying to wounded tissue a composition in the form of a dry powder comprising fibrinogen in an amount which forms a fibrin matrix in the presence of thrombin and Ca++ and aqueous solution;

• providing to the composition thrombin, Ca++ and aqueous solution in amounts which form a fibrin matrix in the presence of fibrinogen;
• wherein said composition is free of collagen;
• wherein said aqueous solution is provided to the dry powder composition at the site of the wounded tissue;
• and further wherein said composition contains no added fibrinolysis inhibitor.

A method for forming a supplemented fibrin matrix, said method comprising applying to wounded tissue a composition in the form of a dry powder comprising an effective amount of at least one supplement and fibrinogen in an amount which forms a fibrin matrix in the presence of thrombin, Factor XIII, Ca++ and aqueous solution;

• and
• providing to the composition thrombin, Factor XIII, Ca++ and aqueous solution in amounts which form a fibrin matrix in the presence of fibrinogen;
• wherein said aqueous solution is provided to the dry powder composition at the site of the wounded tissue;
• wherein said composition is free of collagen;
• wherein said composition contains no added fibrinolysis inhibitor;
• and wherein said supplement is releasable from said fibrin matrix upon dissolution of the clot into the external environment of use for a sustained period, further wherein the amount of said supplement in said composition is greater than the amount of said supplement which is soluble in the fibrin matrix, and
• further wherein said sustained period is greater than the period obtained according to simple diffusion kinetics.