Enveloped virus vaccine and method for production
AssigneeBaxter Healthcare S.A.
InventorsOtfried Kistner, Manfred Reiter, Axel Bruehmann, Noel Barrett, Wolfgang Mundt, Friedrich Dorner
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Abstract
The present invention provides methods of production of a purified enveloped virus antigen. In particular, it provides purified Ross River Virus (RRV) antigens, and vaccines comprising purified, inactivated Ross River Virus (RRV) antigen.
Claims16
A method for production of purified Ross River Virus antigen, comprising the steps of infecting a cell culture with Ross River Virus;
- incubating said cell culture to propagate said virus;
- harvesting the virus produced;
- filtering the harvested virus with a first filter having a pore size of between 0.3 and 1.5 μm;
- filtering the harvested virus with a second filter having a pore size of between 0.1 and 0.5 μm;
- and purifying the virus antigen.
A method far the production of a purified Ross River Virus preparation, comprising the steps of infecting a cell culture with Ross River Virus;
- incubating said cell culture to propagate said virus;
- harvesting the virus produced;
- filtering the harvested virus with a first filter having a pore size of between about 0.3 and about 1.5 μm;
- filtering the harvested virus with a. Second filter having a pore size of between 0.1 and 0.5 μm;
- treating the filtered virus with a nucleic acid degrading agent;
- and purifying the virus.
A method for production of an immunogenic composition comprising purified, inactivated Ross River Virus antigen, comprising the steps of infecting a cell culture with Ross River Virus;
- incubating said cell culture to propagate said virus;
- harvesting the virus produced;
- filtering the harvested virus with a first filter having a pore size of between about 0.3 and about 1.5 μm;
- filtering the harvested virus with a second filter having a pore size of between 0.1 and 0.5 μm;
- treating the filtered virus with a nucleic acid degrading agent and a virus inactivating agent;
- purifying the virus;
- and formulating the purified virus in an immunogenic composition.
Dependencies are inferred from the claim text.