Lymphoid tissue-specific cell production from hematopoietic progenitor cells in three-dimensional devices
AssigneeCytomatrix, LLC
InventorsMark J. Pykett, Michael Rosenzweig, David T. Scadden, Mark C. Poznansky
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Abstract
The invention relates to a method for lymphoid tissue-specific cell production from hematopoietic progenitor cells in unique, three-dimensional culture devices, in the presence of antigen presenting cells and lymphoreticular stromal cells, and in the absence of exogenously added growth factors. The resulting lymphoid tissue-specific cells may be isolated at any sequential stage of differentiation and further expanded. The lymphoid tissue-specific cells also may be genetically altered at any stage of the process.
Claims53
A method for in vitro production of lymphoid tissue-specific cells, comprising:
- introducing an amount of hematopoietic progenitor cells, an amount of antigen presenting cells, and an amount of lymphoreticular stromal cells, into a porous, solid matrix having interconnected pores of a pore size sufficient to permit the hematopoietic progenitor cells, the antigen presenting cells, and the lymphoreticular stromal cells to grow throughout the matrix,
- wherein the lymphoreticular stromal cells are derived from at least one lymphoid soft tissue selected from the group consisting of spleen, liver, lymph node, skin, tonsil and Peyer's patches, and combinations thereof, and the amount of the lymphoreticular stromal cells is sufficient to support the growth and differentiation of the hematopoietic progenitor cells, and
- co-culturing the hematopoietic progenitor cells, antigen presenting cells, and lymphoreticular stromal cells and thereby producing the lymphoid tissue-specific cells,
- wherein the lymphoid tissue-specific cells are T-cells.
Dependencies are inferred from the claim text.