Recombinant viral nucleic acids

A recombinant viral nucleic acid constructed from a virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant viral nucleic acid comprising:

• a first viral subgenomic promoter;
• a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter;
• a second viral subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said virus and whose transcription is regulated by the second viral subgenomic promoter;
• wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other, and wherein the second viral subgenomic promoter is downstream of the first subgenomic promoter.

A recombinant animal viral nucleic acid constructed from an animal virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:

• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other, and wherein the second viral subgenomic promoter is upstream of the first subgenomic promoter.

A recombinant animal viral nucleic acid constructed from an animal virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:

• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other, and wherein the second viral subgenomic promoter is downstream of the first subgenomic promoter.

A process for transcribing a nucleic acid sequence in an animal cell culture comprising:

• (a) infecting an animal cell culture with a recombinant animal viral nucleic acid selected from an animal virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:
• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for an animal viral coal protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal viral subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other thereby enabling the recombinant animal viral nucleic acid to transcribe the second nucleic acid sequence in an animal cell culture, and wherein the second viral subgenomic promoter is upstream of the first subgenomic promoter;
• and
• (b) growing the infected animal cell culture wherein the second nucleic acid sequence is transcribed.

A process for transcribing a nucleic acid sequence in an animal cell culture comprising:

• (a) infecting an animal cell culture with a recombinant animal viral nucleic acid selected from an animal virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:
• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for an animal viral coal protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal viral subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other thereby enabling the recombinant animal viral nucleic acid to transcribe the second nucleic acid sequence in an animal cell culture, and wherein the second viral subgenomic promoter is downstream of the first subgenomic promoter;
• and
• (b) growing the infected animal cell culture wherein the second nucleic acid sequence is transcribed.

A process for transcribing a nucleic acid sequence in an animal cell culture comprising:

• (a) infecting an animal cell culture with a recombinant animal viral nucleic acid selected from a (+) sense, single stranded RNA animal virus possessing a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:
• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for an animal viral coat protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal vital subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said (+) sense, single stranded RNA animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second animal viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other thereby enabling the recombinant animal viral nucleic acid to transcribe the second nucleic acid sequence in an animal cell culture and wherein the second viral subgenomic promoter is upstream of the first subgenomic promoter;
• and
• (b) growing the infected animal cell culture wherein the second nucleic acid sequence is transcribed;
• wherein a gene product is produced from the transcription of the second nucleic acid sequence, the gene product being selected from the group consisting of IL-1, IL-2, IL-3, IL-4, IL-S, IL-6, IL-7, IL-8, IL-9, IL-9, IL-11, IL-12, EPO, G-CSF, GM-CSF, hPG-CSF, M-CSF, Factor VIII, Factor IX, tissue plasminogen activator, human growth hormone, receptors, receptor antagonists, antibodies, neuro-polypeptides, melanin, lipase, hormones, pharmaceuticals, antibiotics, vaccines, and insulin within the animal cell culture.

A process for transcribing a nucleic acid sequence in an animal cell culture comprising:

• (a) infecting an animal cell culture with a recombinant animal viral nucleic acid selected from a (+) sense, single stranded RNA animal virus possessing a naturally occurring subgenomic promoter, the recombinant animal viral nucleic acid comprising:
• a first animal viral subgenomic promoter;
• a nucleic acid sequence that codes for an animal viral coat protein whose transcription is regulated by the first animal viral subgenomic promoter;
• a second animal vital subgenomic promoter;
• and
• a second nucleic acid sequence which is non-native to said (+) sense, single stranded RNA animal virus and whose transcription is regulated by the second animal viral subgenomic promoter;
• wherein the first and second animal viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other thereby enabling the recombinant animal viral nucleic acid to transcribe the second nucleic acid sequence in an animal cell culture and wherein the second viral subgenomic promoter is downstream of the first subgenomic promoter;
• and
• (b) growing the infected animal cell culture wherein the second nucleic acid sequence is transcribed;
• wherein a gene product is produced from the transcription of the second nucleic acid sequence, the gene product being selected from the group consisting of IL-1, IL-2, IL-3, IL-4, IL-S, IL-6, IL-7, IL-8, IL-9, IL-9, IL-11, IL-12, EPO, G-CSF, GM-CSF, hPG-CSF, M-CSF, Factor VIII, Factor IX, tissue plasminogen activator, human growth hormone, receptors, receptor antagonists, antibodies, neuro-polypeptides, melanin, lipase, hormones, pharmaceuticals, antibiotics, vaccines, and insulin within the animal cell culture.