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AssigneesPresident and Fellows of Harvard College, The Regents of the University of California
InventorsRalph H. Lambalot, Amy M. Gehring, Ralph Reid, Christopher T. Walsh
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The invention pertains to isolated phosphopantetheinyl transferases, such as the E. coli acyl carrier protein synthase, which transfer a phosphopantetheinyl group onto a substrate. The enzyme can be purified from a natural source, produced recombinantly, or synthetically. Accordingly, the invention provides compositions and kits including phosphopantetheinyl transferases and host cells expressing phosphopantetheinyl transferases. The invention also provides nucleic acids encoding phosphopantetheinyl transferases and vectors comprising such nucleic acids. The invention further provides methods for phosphopantetheinylating a substrate in vitro or in vivo and methods for producing antibiotics in vitro or in vivo.
A method for phosphopantetheinylating a substrate in a cell, comprising transforming the cell with a first nucleic acid molecule comprising an expressible form of a nucleotide sequence encoding a heterologous Bacillus phosphopantetheinyl transferase, and a second nucleic acid molecule comprising an expressible form of a nucleotide sequence encoding a heterologous protein which is a substrate of the phosphopantetheinyl transferase, such that phosphopantetheinylation of the substrate in the cell occurs, wherein the heterologous Bacillus phosphopantetheinyl transferase comprises:
A method for phosphopantetheinylating a substrate in a cell, comprising transforming the cell with a first nucleic acid molecule comprising an expressible form of a nucleotide sequence encoding a heterologous Bacillus phosphopantetheinyl transferase comprising an amino acid sequence selected from the group consisting of:
Dependencies are inferred from the claim text.