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US 7,192,708 B2Granted
Nucleic acid enzyme biosensors for ions
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Jing Li
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Abstract
A method of detecting the presence of an ion includes contacting a nucleic acid enzyme with a sample suspected of containing the ion, where the enzyme contains a ribonucleotide and is dependent on the ion to produce a product from a substrate, and measuring an amount of the product produced. The ion is Pb2+, and is in the presence of other ions.
Claims69
A method of detecting the presence of an ion, comprising:
- (a) contacting a nucleic acid enzyme, wherein the enzyme is dependent on the ion to produce a product from a substrate, with a sample suspected of containing the ion;
- and
- (b) measuring an amount of the product produced;
- wherein the ion is in the presence of other ions, and the ion is Pb2+;
- and
- wherein the substrate comprises a fluorophore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore.
3 more elements
A method of detecting the presence of an ion, comprising:
- (a) contacting a nucleic acid enzyme, wherein the enzyme is dependent on the ion to produce a product from a substrate, with a sample suspected of containing the ion;
- and
- (b) measuring an amount of the product produced;
- wherein the ion is in the presence of other ions, and the ion is Pb2+;
- the substrate comprises a fluorophore and the enzyme comprises a quencher of the fluorophore, or the enzyme comprises a fluorophore and the substrate comprises a quencher of the fluorophore;
- the nucleic acid enzyme comprises a deoxyribozyme;
- the nucleic acid enzyme and the substrate comprise separate nucleic acid strands;
- and the measuring comprises a measurement of fluorescence.
5 more elements
Dependencies are inferred from the claim text.