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US 7,192,560 B2Granted
Methods and devices for removal of organic molecules from biological mixtures using anion exchange
Assignee3M Innovative Properties Company
InventorsRanjani V. Parthasarathy, Raj Rajagopal, Erin E. Olson, Frank J. Beissel, IV, William Bedingham, Barry W. Robole
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Abstract
Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.
Claims47
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising an anion exchange material partially coated with a negatively charged polymer;
- providing a biological sample mixture comprising small negatively charged organic molecules having a molecular weight of less than about 6,000;
- wherein the biological sample mixture is selected from the group consisting of a nucleic acid amplification reaction mixture and a nucleic acid labeling reaction mixture;
- and
- contacting the biological sample mixture with the surface comprising the anion exchange mnaterial to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
6 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising an anion exchange material partially coated with a negatively charged polymer;
- providing a biological sample mixture;
- and
- contacting the biological sample mixture with the surface comprising an anion exchange material partially coated with a negatively charged polymer to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
5 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte;
- providing a biological sample mixture;
- and
- contacting the biological sample mixture with the surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
5 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte;
- providing a biological sample mixture;
- and
- contacting the biological sample mixture with the surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture;
- wherein the biological sample mixture comprises a nucleic acid amplification reaction mixture.
6 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising an anion exchange material partially coated with a negative polymer;
- providing a biological sample mixture in the at least one process array, wherein the biological sample mixture comprises small negatively charged organic molecules having a molecular weight of less than about 6,000;
- and
- transferring the biological sample mixture within the at least one process array, wherein the biological sample mixture and the surface comprising an anion exchange material remain in contact for a sufficient time to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
5 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising an anion exchange material partially coated with a negatively charged polymer;
- providing a biological sample mixture in the at least one process array;
- and
- transferring the biological sample mixture within the at least one process array, wherein the biological sample mixture and the surface comprising an anion exchange material partially coated with a negatively charged polymer remain in contact for a sufficient time to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
5 more elements
A method of removing small negatively charged organic molecules from a biological sample mixture, the method comprising:
- providing a device comprising a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising quaternary ammonium ions partially coaxed with a negatively charged polyelectrolyte;
- providing a biological sample mixture in the at least one process array;
- and
- transferring the biological sample mixture within the at least one process array, wherein the biological sample mixture and the surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte remain in contact for a sufficient time to remove at least a portion of the small negatively charged organic molecules from the biological sample mixture.
5 more elements
A device comprising:
- a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising an anion exchange material partially coated with a negatively charged polymer;
- and
- wherein the device is operable to remove small negatively charged organic molecules from the biological sample mixture.
4 more elements
A device comprising:
- a plurality of process arrays, wherein each process array of the plurality of process arrays comprises:
- a plurality of process chambers, each of the process chambers defining a volume for containing a biological sample mixture;
- and
- at least one distribution channel connecting the plurality of process chambers of the array;
- wherein at least one of the process arrays comprises a surface comprising quaternary ammonium ions partially coated with a negatively charged polyelectrolyte;
- and
- wherein the device is operable to remove small negatively charged organic molecules from the biological sample mixture.
4 more elements
Dependencies are inferred from the claim text.