Electrochemiluminescence from acridan compounds
AssigneeLumigen, Inc.
InventorsHashem Akhavan-Tafti, Robert Wilson, David Jorge Schiffrin
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Abstract
Methods are disclosed for producing electrochemiluminescence by electrochemically oxidizing an acridan compound at an electrode in the presence of a peroxide. Maintaining a sufficiently positive potential results in continuous oxidation of the acridan compound to an acridinium compound which reacts with peroxide to produce the luminescence. Light emission can be reversibly and repeatedly cycled on and off by sweeping the potential between two values. The acridan compounds can be provided with a labeling group for linking to an analyte or analyte binding partner. The present electrochemiluminescent reaction can find use in assay methods for detecting analytes by immunoassays and nucleic acid assays.
Claims22
A method for producing electrochemiluminescence comprising:
- a) providing a solution of an acridan compound and a peroxide, wherein the acridan compound has the formula
- wherein R1 to R4 are independently selected from hydrogen and organic groups containing from 1 to 50 non-hydrogen atoms selected from C, N, O, S, P and halogen atoms, Z is selected from O, S and NR5, R5 is selected from substituted or unsubstituted alkyl, substituted or unsubstituted aryl, alkylsulfonyl and arylsulfonyl groups;
- and
- b) electrochemically oxidizing the acridan compound at a electrode to form an oxidized intermediate compound wherein the oxidized intermediate compound reacts with the peroxide provided in the solution to produce the chemiluminescence.
A method for producing electrochemiluminescence comprising:
- a) providing a solution of an acridan compound and a peroxide, wherein the acridan compound has the formula:
- wherein R1 to R4 are independently selected from O, S and NR5, R5 is selected from substituted or unsubstituted alkyl, substituted or unsubstituted aryl, alkylsulfonyl and arylsulfonyl groups, R1 to R4 are independently selected from hydrogen and organic groups containing from 1 to 50 non-hydrogen atoms selected from C, N, O, S, P and halogen atoms, and X is selected from hydrogen and organic groups containing from 1 to 50 non-hydrogen atoms selected from C, N, O, S, P and halogen atoms;
- b) electrochemically oxidizing the acridan compound at a electrode to form an oxidized intermediate compound wherein the oxidized intermediate compound reacts with the peroxide provided in the solution to produce the chemiluminescence.
Dependencies are inferred from the claim text.