3-hydroxypropionic acid and other organic compounds
AssigneeCargill, Incorporated
InventorsRavi R. Gokarn, Olga V. Selifonova, Holly Jean Jessen, Steven John Gort, Thorsten Selmer, Wolfgang Buckel
Life of this patent
Estimating the term…
Abstract
Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.
Claims10
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) a sequence set forth in SEQ ID NO:18;
- (b) a portion of SEQ ID NO:
- 18 having 30 or more contiguous amino acid residues of SEQ ID NO:
- 18, wherein the portion of SEQ ID NO:
- 18 can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity;
- (c) the sequence set forth in SEQ ID NO:
- 18, containing 5 conservative substitutions;
- and
- (d) the sequence set forth in SEQ ID NO:
- 18, containing 10 conservative substitutions.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 18.
An isolated polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:
- 18.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 18 containing 5 conservative substitutions.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 18 containing 10 conservative substitutions.
An isolated polypeptide comprising at least 95% sequence identity to SEQ ID NO:
- 18, wherein the polypeptide can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.
An isolated polypeptide comprising a portion of SEQ ID NO:
- 18 having 30 or more contiguous amino acid residues of-SEQ ID NO:
- 18, wherein the portion of SEQ ID NO:
- 18 can function as an E2 alpha polypeptide subunit of an enzyme having lactyl-CoA dehydratase activity.
Dependencies are inferred from the claim text.