Ex vivo and in vivo expression of the thrombomodulin gene for the treatment of cardiovascular and peripheral vascular diseases
AssigneeBiovec, LLC
InventorsLakshman R. Sehgal, Jonathan Wong
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Abstract
The present invention relates to methods and compositions for treatment of cardiovascular and peripheral vascular diseases using ex vivo and in vivo gene delivery technologies. One aspect of the present invention relates to a method for treating a vascular disease by introducing a DNA sequence encoding a TM protein or its variant into a segment of a blood vessel ex vitro using a gutless adenovirus vector. Another aspect of the present invention is to provide a method to deliver a gutless adenovirus vector carrying a DNA sequence encoding a TM protein or its variant using a stent.
Claims14
A method for treating a vascular disease in a mammal, wherein said method comprising the steps of:
- infecting a seament of a blood vessel in vitro using a gutless adenoviral vector which comprises a polynucleotide encoding a thrombomodulin protein having the amino acid sequence of SEQ ID NO:2 and a regulatory element operably linked to said polynucleotide sequence;
- grafting the virus-treated blood vessel in said mammal,
- wherein said thrombomodulin protein is expressed in said virus-treated blood vessel in an amount sufficient to reduce re-occlusion or intimal hyperplasia in the grafted blood vessel.
Dependencies are inferred from the claim text.