Taxol immunoassay
AssigneeSaladax Biomedical Inc.
InventorsSalvatore J. Salamone, Jodi Blake Courtney, Dennis Stocker, Mahmoud Ahmed ElSohly, Waseem Gul
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Abstract
Novel conjugates of taxol and novel taxol immunogens derived from the 9 and 7 positions of taxol and monoclonal antibodies generated by these taxol linked immunogens are useful in immunoassays for the quantification and monitoring of taxol in biological fluids.
Claims17
An immunoassay method for detecting taxol in a sample providing a mixture of a sample, containing an antibody selectively reactive with taxol and not substantially cross-reactive with both 6-α-hydroxypaclitaxel and 3′-p-hydroxypaclitaxel and a conjugate of a carrier or a polyamine polymer with a compound of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a terminal functional group capable of binding to said carrier or said polyamine polymer;
- p is an integer from 0 to 1;
- and
- Ph is phenyl
- or compounds of the formula:
- wherein X, Y and p are as above and B is
- or mixtures thereof,
- causing the taxol in the sample and said conjugate to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of taxol in the sample can be determined.
An antibody which binds selectively to taxol and does not substantially bind to both 6-α-hydroxypaclitaxel and 3′-p-hydroxypaclitaxel.
A kit for determining the presence of taxol in a patient sample comprising reagents in separate containers, the first of said containers containing as a reagents a conjugate of a carrier or a polyamine polymer with a compound selected from the groups consisting of compounds of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a terminal functional group capable of binding to said carrier or said a polyamine polymer
- p is an integer from 0 to 1;
- and
- Ph is phenyl
- or a compound of the formula:
- wherein X, Y and p are as above and B is
- and mixtures thereof;
- and a second container containing an antibody substantially selectively reactive with taxol and not substantially cross-reactive to both 6-α-hydroxypaclitaxel and 3′-p-hydroxypaclitaxel.
Dependencies are inferred from the claim text.