Formulation for topical non-invasive application in vivo

A formulation comprising penetrants being capable of penetrating the pores of a barrier, the average diameter of said pores being smaller than the average diameter of said penetrants, wherein said penetrants can transport agents or enable agent penetration through said pores after said penetrants have entered said pores, wherein the formulation further comprises

• 1) at least one antioxidant in an amount that reduces the increase of oxidation index to less than 100% per 6 months, wherein the antioxidant is selected from the group consisting of between 0.0025 and 0.2 w-% of butylated hydroxyanisol, between 0.0025 and 0.2 w-% of butylated hydroxytoluene, between 0.001 and 2 w-% of tertiary butylhydroquinone, between 0.001 and 2 w-% of propyl gallate, between 0.005 and 5 w-% of tocopherols, between 0.001 and 5 w-% of ascorbic acid esters, between 0.001 and 5 w-% of ascorbic acid, between 0.001 and 5 w-% of sodium bisulphite, between 0.001 and 5 w-% of sodium metabisulphite, between 0.0001 and 2 w-% of thiourea, between 0.01 and 5 w-% of cysteine, between 0.01 and 5 w-% of monothioglycerol, between 0.0005–2 w-% of nordihydroguaiaretic acid, between 0.005 and 5 w-% of glutathione, between 0.001 and 5 w-% of EDTA, and between 0.001 and 5 w-% of citric acid, based on the total weight of the formulation;
• and
• 2) at least one microbiocide in an amount that reduces the bacterial count of 1 million germs added per gram of total mass of the formulation to less than 100 in the case of aerobic bacteria, to less than 10 in the case of entero-bacteria, and to less than 1 in the case of Pseudomonas aeruginosa or Staphilococcus aureus, after a period of 4 days, wherein the microbicide is selected from the group consisting of up to 10 w-% of ethyl alcohol, up to 10 w-% of propyl alcohol, up to 10 w-% of butyl alcohol, up to 10 w-% of benzyl alcohol, between 0.3–0.6 w-% of chlorobutanol, between 0.05–0.2 w-% of parabens, between 0.05–0.2 w-% of methyl paraben, between 0.002–0.02 w-% of propyl paraben, between 0.05–0.2 w-% of sorbic acid, between 0.1–0.5 w-% of benzoic acid, between 0.1–0.3 w-% of phenols, between 0.1–0.3 w-% of triclosan, and between 0.01–0.05 w-% of chlorhexidine, based on the total weight of the formulation;
• wherein the agent is selected from corticosteroids and the relative content of corticosteroids is above 0.1 weight-%, relative to total dry mass of the formulation.