Virus vectors and methods of making and administering the same
AssigneeThe University of North Carolina at Chapel Hill
InventorsJoseph E. Rabinowitz, Richard Jude Samulski, Weidong Xiao
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Abstract
The present invention provides genetically-engineered parvovirus capsids and viruses designed to introduce a heterologous gene into a target cell. The parvoviruses of the invention provide a repertoire of vectors with altered antigenic properties, packaging capabilities, and/or cellular tropisms as compared with current AAV vectors.
Claims54
A hybrid virus particle comprising:
- a parvovirus capsid;
- and
- a nucleic acid comprising at least one adeno-associated virus (AAV) serotype 2 inverted terminal repeat packaged within said parvovirus capsid, subject to the proviso that if said parvovirus capsid is an AAV capsid, the serotypes of said AAV capsid and said at least one AAV inverted terminal repeat are different.
A cell comprising a vector comprising:
- parvovirus cap genes,
- adeno-associated virus (AAV) rep genes, and
- a nucleic acid comprising at least one AAV serotype 2 inverted terminal repeat,
- subject to the proviso that if said parvovirus cap genes are AAV cap genes, said at least one AAV inverted terminal repeat is of a different AAV serotype than said cap genes.
A cell comprising parvovirus cap genes and adeno-associated virus (AAV) rep genes stably integrated into the genome of the cell, subject to the proviso that if said parvovirus cap genes are AAV cap genes, the serotypes of said AAV cap genes and said AAV rep genes are different.
A method of producing a hybrid virus particle, comprising:
- providing a cell with adeno-associated virus (AAV) rep genes, parvovirus cap genes, a nucleic acid comprising at least one AAV serotype 2 inverted terminal repeat, and helper functions for generating a productive AAV infection;
- subject to the proviso that if the parvovirus cap genes are AAV cap genes, the serotypes of the AAV cap genes and the at least one AAV inverted terminal repeat are different, and
- allowing assembly of the hybrid virus particles.
Dependencies are inferred from the claim text.