Magnetic particles and methods of producing coated magnetic particles
AssigneeSkold Technology
InventorCarl Nelson Skold
Life of this patent
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Abstract
An improved method for separating materials is provided, using colloidal, magnetizable aggregates, optionally silanized, and coated with a one or more layers of novel polysaccharide derivatives. Materials separated by the aggregates of the invention include inorganic and organic molecules, viruses, organelles, and cells. The invention also relates to a kit for separating such materials. The separated materials are useful in analytical and preparative or in diagnostic and therapeutic techniques.
Claims19
A method of separating a target material from a liquid mixture, comprising:
- forming and at least substantially purifying aggregates having a plurality of crystallites of a magnetizable metal oxide;
- coating the formed and at least substantially purified aggregates with a polysaccharide material to form coated aggregates, wherein the polysaccharide material includes at least one pendant carboxyl group directly attached to a linker which is directly attached to a polysaccharide, wherein the linker has at least one heteroatom for every three carbon atoms in the linker;
- treating the coated aggregates by attaching a specific binding member having a binding affinity for the target material to the polysaceharide material via the carboxyl group to form treated aggregates;
- combining the treated aggregates with the liquid mixture containing the target material for a sufficient time for the target material to bind to the specific binding member;
- applying a magnetic field to the combination of the treated aggregates and the liquid mixture;
- and
- separating the treated aggregates, including the target material bound thereto, from the liquid mixture, using the magnetic field.
A method of separating a target material from a liquid mixture, comprising:
- forming and at least substantially purifying aggregates of two or more crystallites of a magnetizable metal oxide;
- coating the formed and at least substantially purified aggregates with a polysaccharide material to form coated aggregates, wherein the polysaccharide material includes at least one pendant carboxyl group attached to the polysaccharide through a linker having at least one heteroatom for every three carbon atoms in the linker, wherein the pendant carboxyl group is introduced by reaction with chloroethoxyethoxyacatic acid and base;
- attaching a coupling group having an affinity for the target material to the polysaccharide material via the carboxyl group, to form treated aggregates;
- combining the treated aggregates with the liquid mixture containing the target material for a sufficient time for the target material to bind to the polysaccharide material;
- applying a magnetic field to the combination of the treated aggregates and the liquid mixture;
- and
- separating the treated aggregates, including the target material bound thereto, from the liquid mixture, using the magnetic field.
Dependencies are inferred from the claim text.