Neisseria meningitidis serogroup B glycoconjugates and methods of producing the same
AssigneeChiron Corporation
InventorRobert Seid
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Abstract
The present invention pertains generally to novel Neisseria meningitidis serogroup B glycoconjugates. More particularly, the invention pertains to glycoconjugates formed from a Neisseria meningitidis serogroup B capsular oligosaccharide derivative (MenB OS derivative) in which sialic acid residue N-acetyl groups are replaced with N-acyl groups. The invention also pertains to vaccine formulations containing the glycoconjugates, methods of making the vaccine formulations, and methods of using the vaccine formulations to treat or prevent Neisseria meningitidis serogroup B or E. coli K1 disease in a mammalian subject.
Claims7
A substantially homogenous sized Neisseria meningitidis serogroup B capsular oligosaccharide (MenB OS)/CRM197 toxoid glycoconjugate produced by a method comprising:
- (a) providing a heterogenous population of MenB OS in which sialic acid residue N-acetyl groups are replaced with saturated N-propionyl groups;
- (b) obtaining a substantially homogenous sized group of MenB OS from the population of step (a) wherein said MenB OS have an average degree of polymerization (Dp) of about 12 to 18;
- (c) covalently attaching a C3–C16 long-chain aliphatic lipid to the nonreducing end of the MenB OS obtained in step (b);
- (d) introducing a reactive group at the reducing end of the MenB OS obtained in step (b) to provide single end-activated MenB OS of said DP;
- and
- (e) covalently attaching the single end-activated MenB OS obtained in step (d) to a CRM197 toxoid carrier molecule to provide the substantially homogenous sized MenB OS/CRM197 toxoid glycoconjugate.
A substantially homogenous sized Neisseria meningitidis serogroup B capsular oligosaccharide (MenB OS) glycoconjugate produced by a method comprising:
- (a) providing a heterogenous population of MenB OS in which sialic acid residue N-acetyl groups are replaced with N—C3–C8 acyl groups;
- (b) obtaining a substantially homogenous sized group of MenB OS from the population of step (a) wherein said group of MenB OS has an average degree of polymerization (Dp) of about 10 to 20;
- (c) introducing a reactive group at the reducing end of the MenB OS obtained in step (b) to provide single end-activated MenB OS of said DP;
- and
- (d) covalently attaching the single end-activated MenB OS obtained in step (c) to a protein carrier molecule to provide the substantially homogenous sized MenB OS glycoconjugate.
Dependencies are inferred from the claim text.