US 7,169,289 B2Granted

Electrochemical detection method and device

Assigneenovember Aktiengesellschaft Gesellschaft fur Molekulare Medizin

InventorsJürgen Schülein, Björn Grassl, Jörg Hassmann

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Abstract

A device for the electrochemical detection of at least one biochemical molecule contained in a liquid from a group of predetermined biochemical molecules includes a holder (1) for taking up the liquid, with at least one reference electrode (RE) and at least one counterelectrode (GE) and a multiplicity of working electrodes (AE1, AE2, AE3). At least one working electrode is provided for the detection of each biochemical molecule, and is coated with a molecule that is complementary to the respective biochemical molecule, so that the biochemical molecules can be detected simultaneously. The device includes a potentiostat (P) for generating a predetermined voltage profile between the working electrodes and the reference electrode, a current/voltage converter (S1, S2, S3) connected downstream of each of the working electrodes for holding all of the working electrodes at the same potential, and a measurement device (Ad) for measuring the currents flowing through the working electrodes.

Claims12

Dependent claims

A device for the electrochemical detection of at least one type of a biochemical molecule contained in a liquid from a group of predetermined biochemical molecules of different types, comprising:

a means (1) for taking up the liquid, said means having at least one reference electrode (RE) and at least one counterelectrode (GE) and more than two working electrodes (AE1, AE2, AE3), with at least one working electrode (AE1, AE2, AE3) being provided for the detection of each type of a biochemical molecule, said working electrode being coated with a molecule that is complementary to the biochemical molecule to be detected, so that biochemical molecules of different types can be detected simultaneously,
a current/voltage converter (S1, S2, S3) being connected downstream of each of the working electrodes (AE1, AE2, AE3) and having a first operational amplifier (OP1) and a first resistor (R1), the current/voltage converters (S1, S2, S3) holding all of the working electrodes (AE1, AE2, AE3) at the same potential,
a means (AD) for measuring the currents flowing through the working electrodes (AE1, AE2, AE3), and
a potentiostat (P) for generating a predetermined voltage profile which is variable during the measurement between the working electrodes (AE1, AE2, AE3) and the reference electrode (RE),
said potentiostat (P) comprising a second operational amplifier (OP2) and a second resistor (R2), and a third operational amplifier (OP3) and a third resistor (R3),
with the third operational amplifier (OP3), to whose output the counterelectrode (GE) is connected and whose inverting input (0P3−) is connected via the second resistor (R2) to the output of the second operational amplifier (OP2), being connected via the third resistor (R3) to a device for generating a selectable desired voltage, with the noninverting input (0P3+) of the third operational amplifier (OP3) being grounded.

3 more elements

A method for electrochemical detection of at least one type of a biochemical molecule contained in a liquid from a group of predetermined biochemical molecules of different types, comprising the steps of:

a) providing a means (1) for taking up the liquid, the means (1) having at least one counterelectrode (GE) and a reference electrode (RE) and more than two working electrodes (AE1, AE2, AE3), with at least one working electrode (AE1, AE2, AE3) being provided for the detection of each biochemical molecule, said working electrode being coated with a molecule that is complementary to the biochemical molecule to be detected, so that biochemical molecules of different types can be detected simultaneously,
b) bringing the liquid into contact with the working (AE1, AE2, AE3), counter- (GE) and reference electrodes (RE),
c) simultaneously applying a predetermined voltage profile which is variable during the measurement between the working electrodes (AE1, AE2, AE3) and the reference electrode (RE),
while regulating said voltage between the working electrodes (AE1, AE2, AE3) and the reference electrode (RE) with a potentiostat (P) for generating a predetermined voltage profile which is variable during the measurement between the working electrodes (AE1, AE2, AE3) and the reference electrode (RE), said potentiostat (P) comprising a second operational amplifier (OP2) and a second resistor (R2), and a third operational amplifier (OP3) and a third resistor (R3), with the third operational amplifier (OP3), to whose output the counterelectrode (GE) is connected and whose inverting input (OP3−) is connected via the second resistor (R2) to the output of the second operational amplifier (OP2), being connected via the third resistor (R3) to a device for generating a selectable desired voltage, with the noninverting input (OP3+) of the third operational amplifier (OP3) being grounded, and
d) measuring the currents flowing through the working electrodes (AE1, AE2, AE3), all of the working electrodes (AE1, AE2, AE3) being held at the same potential during the measurement.

2 more elements

Dependencies are inferred from the claim text.