Polypeptide
AssigneeDanisco A/S
InventorsKarsten Matthias Kragh, Harm Mulder, Steffen Petersen, Helle Fomsgaard, Oene Robert Veltman
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Abstract
We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
Claims24
A PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises a proline substitution at position 141 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO:
- 1, in which the PS4 variant polypeptide has at least 95% homology to SEQ ID NO:
- 1 and non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions.
A PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises a proline substitution at position 141, or an alanine substitution at position 223, or both, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO:
- 1, in which the PS4 variant polypeptide has at least 95% homology to SEQ ID NO:1 and non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions.
A polypeptide sequence comprising a sequence of a non-maltogenic exoamylase which has been mutated to include a proline substitution at an amino acid position 141 or an alanine substitution at position 223, or both, with reference to a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO:
- 1.
A PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide being a non-maltogenic exoamylase from Pseudomonas saccharophilia, the variant having a sequence shown as SEQ ID NO:
- 1 and non-maltogenic exoamylase activity and comprising a proline substitution at position 141, or a polypeptide which has at least 95% similarity thereto, non-maltogenic exoamylase activity and a proline residue at position 141.
A polypeptide obtainable by altering the sequence of a non-maltogenic exoamylase from Pseudomonas saccharophilia having a sequence shown as SEQ ID NO:
- 1, or a polypeptide which has at least 95% similarity thereto, by introducing a proline substitution at position 141.
A combination of a PS4 variant polypeptide according to any preceding claim, together with Novamyl, or a variant, homologue, or mutants thereof which has alpha amylase activity, or a composition comprising such.
Dependencies are inferred from the claim text.