Direct selection of cells by secretion product

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lipid anchor.

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is an antibody, or an antigen-binding fragment thereof.

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method, and wherein said capture moiety is coupled to said cells through direct chemical coupling of the capture moiety to components on the cell surface, optionally through a linking moiety.

A composition comprising cells positively identified based on a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein said product is labeled with a label moiety, wherein said capture moiety is an antibody or antigen-binding fragment thereof.

A composition comprising cells positively identified based on a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein said product is labeled with a label moiety, wherein the label moiety is fluorochromated.

A composition comprising cells positively identified based on a cytokine secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said cytokine secreted by said cells is bound to said capture moiety, and wherein said cytokine is labeled with a label moiety, and wherein said cytokine is IFNγ, IL1, IL2, IL4, IL10, IL12, TGFβ, TNF, GMCSF, and SCF.

A composition comprising cells positively identified based on a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein said product is labeled with a label moiety, wherein said capture moiety is coupled to said cells through direct chemical coupling of the capture moiety to components on the cell surface, optionally through a linking moiety wherein said linking moiety includes a branched polymer.

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, and wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method further comprising the step of positively separating said cells labeled with said product.

A method to positively separate cells based on a product secreted by the cells, comprising the steps of:

• a) culturing cells coupled to a capture moiety under conditions wherein a product is secreted, wherein said product secreted by said cells specifically binds to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method, and wherein said product is labeled with a label moiety;
• and
• b) positively separating said cells labeled with said product.

A method to positively separate cells based on a product secreted by the cells comprising separating cells labeled with the product, wherein said cells have been coupled to a capture moiety that specifically binds a product secreted by said cells and wherein said cells have been cultured under conditions wherein the product is secreted and bound to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method and wherein said product is labeled with a label moiety.

A method to positively separate cells based on a product secreted by the cells comprising separating cells labeled with the product, wherein said cells have been coupled to a capture moiety that specifically binds a product secreted by said cells and wherein said cells have been cultured under conditions wherein the product is secreted and bound to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, and wherein said capture moiety is coupled to said cells through an anchoring moiety.

A method to positively separate cells based on a product secreted by the cells comprising separating cells labeled with the product, wherein said cells have been coupled to a capture moiety that specifically binds a product secreted by said cells and wherein said cells have been cultured under conditions wherein the product is secreted and bound to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, and wherein said capture moiety is coupled to said cells through direct chemical coupling of the capture moiety to components on the cell surface, optionally through a linking moiety.

A method to determine the amount of product produced per cell in a population of cells, comprising the steps of:

• a) culturing a cell population, wherein said cells of said population are coupled to a capture moiety which specifically binds a product secreted by said cells, under conditions wherein the product is secreted and bound to said capture moiety thereby producing cells labeled with said product;
• and
• b) determining the amount of product produced per cell labeled with said product.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, and wherein said product is optionally labeled with a label moiety, and wherein said cells are not lysed during said method.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is optionally labeled with a label moiety, wherein said cells are not lysed during said method, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lipid anchor.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is optionally labeled with a label moiety, wherein said cells are not lysed during said method, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is an antibody, or an antigen-binding fragment thereof.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is optionally labeled with a label moiety, wherein said cells are not lysed during said method, and wherein said capture moiety is coupled to said cells through direct chemical coupling of the capture moiety to components on the cell surface, optionally through a linking moiety.

A composition comprising cells labeled with a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein optionally, said product is labeled with a label moiety, wherein said capture moiety is an antibody or antigen-binding fragment thereof.

A composition comprising cells labeled with a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein optionally, said product is labeled with a label moiety wherein said capture moiety is coupled to said cells through an anchoring moiety wherein said anchoring moiety is an antibody or an antigen-binding fragment thereof.

A The composition comprising cells labeled with a product secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said product secreted by said cells is bound to said capture moiety, and wherein optionally, said product is labeled with a label moiety

• wherein said capture moiety is coupled to said cells through direct chemical coupling of the capture moiety to components on the cell surface, optionally through a linking moiety wherein said linking moiety comprises a branched polymer.

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method,

• wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin.

A method to positively identify cells based on a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells wherein said capture moiety specifically binds the product, thereby labeling cells with said product, wherein said product is labeled with a label moiety, wherein said cells are not lysed during said method,

• wherein said capture moiety is an antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody to the cell.

A method to positively separate cells based on a product secreted by the cells, comprising the steps of:

• a) culturing cells coupled to a capture moiety under conditions wherein a product is secreted, wherein said product secreted by said cells specifically binds to said capture moiety, thereby producing cells labeled with said product wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin;
• and
• b) positively separating said cells labeled with said product.

A method to positively separate cells based on a product secreted by the cells, comprising the steps of:

• a) culturing cells coupled to a capture moiety under conditions wherein a product is secreted, wherein said product secreted by said cells specifically binds to said capture moiety, thereby producing cells labeled with said product wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, wherein said capture moiety is antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody or antigen binding fragment thereof to the cell;
• and
• b) positively separating said cells labeled with said product.

A method to positively separate cells based on a product secreted by the cells comprising separating cells labeled with the product, wherein said cells have been coupled to a capture moiety that specifically binds a product secreted by said cells and wherein said cells have been cultured under conditions wherein the product is secreted and bound to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin.

A method to positively separate cells based on a product secreted by the cells comprising separating cells labeled with the product, wherein said cells have been coupled to a capture moiety that specifically binds a product secreted by said cells and wherein said cells have been cultured under conditions wherein the product is secreted and bound to said capture moiety, thereby producing cells labeled with said product, wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, wherein said capture moiety is an antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody or antigen binding fragment thereof to the cell.

A method to determine the amount of product produced per cell in a population of cells, comprising the steps of:

• a) culturing a cell population, wherein said cells of said population are coupled to a capture moiety which specifically binds a product secreted by said cells, under conditions wherein the product is secreted and bound to said capture moiety thereby producing cells labeled with said product, wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin;
• and
• b) determining the amount of product produced per cell labeled with said product.

A method to determine the amount of product produced per cell in a population of cells, comprising the steps of:

• a) culturing a cell population, wherein said cells of said population are coupled to a capture moiety which specifically binds a product secreted by said cells, under conditions wherein the product is secreted and bound to said capture moiety thereby producing cells labeled with said product, wherein said capture moiety is an antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody or antigen binding fragment thereof to the cell;
• and
• b) determining the amount of product produced per cell labeled with said product.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, and wherein said product is optionally labeled with a label moiety, wherein said cells are not lysed during said method, and wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin.

A method to label cells with a product secreted by the cells, comprising culturing said cells under conditions wherein the product is secreted and bound to a capture moiety coupled to said cells, wherein said capture moiety specifically binds the product, thereby labeling cells with said product, and wherein said product is optionally labeled with a label moiety, wherein said cells are not lysed during said method, wherein said cells are not lysed by said method, wherein said product is labeled with a label moiety, and wherein said capture moiety is an antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody or antigen binding fragment thereof to the cell.

A composition comprising viable cells labeled with a product secreted by said cells,

• wherein said cells are coupled to a capture moiety, said product secreted by said cells is bound to said capture moiety, and
• said product is labeled with a label moiety wherein said capture moiety is an antibody or antigen binding fragment thereof, the antibody or antigen binding fragment thereof is bispecific, and the coupling is through specific binding of the antibody or antigen binding fragment thereof to the cell.

A composition comprising viable cells, wherein said cells are coupled to a capture moiety, wherein a product secreted by said cells is specifically bound to said capture moiety, and wherein said product is labeled with a label moiety that permits the labeled cells to be positively selected based on the presence of said label moiety wherein said capture moiety is coupled to said cells through an anchoring moiety, and wherein the anchoring moiety is a lectin.

A composition comprising viable cells labeled with a cytokine secreted by said cells, wherein said cells are coupled to a capture moiety, wherein said cytokine secreted by said cells is bound to said capture moiety, and wherein said cytokine is labeled with a label moiety.

A composition comprising viable cells, wherein said cells are coupled to a capture moiety, wherein a cytokine secreted by said cells is specifically bound to said capture moiety, and wherein said cytokine is labeled with a label moiety that permits the labeled cells to be positively selected based on the presence of said label moiety.