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US 7,160,684 B2Granted
Methods of selecting compounds for modulation of bladder function
AssigneeWyeth
InventorsThomas M. Argentieri, Jeffrey H. Sheldon
Life of this patent
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Abstract
The present invention involves an assay system and method of selecting compounds useful in the treatment of bladder instability and related bladder conditions through the activation of KCNQ potassium channels in the bladder smooth muscle.
Claims57
A method of screening for a compound for the inhibition of urge urinary incontinence comprising:
- (a) expressing a target KCNQ protein in a host cell;
- and
- (b) detecting activation of said target KCNQ protein in the host cell;
- wherein the detection is performed by measuring the membrane potential of the host cell in the presence or absence of said compound and selecting the compound whose presence causes hyperpolarization in membrane potential of the host cell.
1 more element
A method of screening for a compound for the inhibition of urge urinary incontinence comprising:
- (a) expressing a target KCNQ channel in a host cell;
- and
- (b) detecting activation of said target KCNQ channel in the host cell;
- wherein the detection is performed by measuring the membrane potential of the host cell in the presence or absence of said compound and selecting the compound whose presence causes a change in membrane potential of the host cell.
1 more element
A method of screening for a compound for the inhibition of urge urinary incontinence comprising:
- (a) recombinantly expressing a target KCNQ channel in a host cell;
- (b) measuring membrane potential of the host cell in the presence or absence of the compound;
- and
- (c) selecting the compound whose presence causes hyperpolarization of the host cell.
1 more element
Dependencies are inferred from the claim text.