Method of performing reverse transcription reaction using reverse transcriptase encoded by non-LTR retrotransposable element
AssigneeUniversity of Rochester
InventorsThomas H. Eickbush, Arkadiusz Bibillo
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of preparing a cDNA molecule which includes: contacting an RNA molecule, in the presence of dNTPs, with a non-LTR retrotransposon protein or polypeptide having reverse transcriptase activity under conditions effective for production of a cDNA molecule complementary to the RNA molecule, the contacting being carried out in the absence of a target DNA molecule of the non-LTR retrotransposon protein or polypeptide; and isolating the cDNA molecule. The preferred non-LTR retrotransposon protein or polypeptide is an R2 protein or polypeptide.
Claims32
A method of preparing a cDNA molecule comprising:
- contacting an RNA molecule, in the presence of dNTPs, with a non-LTR retrotransposon protein or polypeptide having reverse transcriptase activity, wherein the non-LTR retrotransposon protein or polypeptide is an R2 protein or polypeptide, under conditions effective for production of a cDNA molecule complementary to the RNA molecule, said contacting being carried out in the absence of a target DNA molecule of the non-LTR retrotransposon protein or polypeptide;
- and
- isolating the cDNA molecule.
Dependencies are inferred from the claim text.