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US 7,141,773 B2Granted
Image focusing in fluorescent imaging
AssigneeBioview Ltd.
InventorsEran Kaplan, Avner Freiberger
Life of this patent
Estimating the term…
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AssigneeBioview Ltd.
InventorsEran Kaplan, Avner Freiberger
Estimating the term…
An imaging apparatus with an autofocus mechanism for obtaining focused images. The apparatus comprises: an objective lens, a focus controller for altering a distance between the objective lens and a sample, an object finder for finding objects of interest within the sample, for example cells, and a light intensity measurement unit which measures light intensity levels of the thus identified objects of interest. The focus control alters the sample-objective distance to maximize the light intensity levels being measured, thereby to arrive at a focus position. Objects of interest may be identified by filtering out large objects and optionally by masking out background regions. The apparatus is useful for microscopy and particularly for fluorescent imaging in which light levels are low and noise is often high.
An imaging apparatus for taking images of samples, the apparatus comprising:
A method of image focusing comprising:
A method of producing a focused image comprising:
An autofocus device for use in imaging, the device comprising:
An autofocus device for a fluorescence imaging microscope, the device comprising an image filter for filtering an image to delineate objects of interest and a light intensity measurement unit associated with a focusing mechanism for focusing by altering a focus distance to maximize a calculated sum of measured light intensity of said filtered image.
Dependencies are inferred from the claim text.